Gram Positive Cocci

Question 1

Another name for spingomyelinase C or Hot cold lysin

Answer 1

Beta hemolysin-responsible for hemolysis in BAP. Beta means complete hemolysis = colorless zone

Question 2

Pyrogenic exotoxins C is also known as _____, which is caused by what agent?

Answer 2

SAU

Question 3

Marker of virulence for SAU

Answer 3

Coagulase- converts fibrinogen to fibrin; for cell attachment

Question 4

Also known as clumping factor

Answer 4

Bound coagulase - detected in slide coagulase. Does not Requires incubation unlike tube method.

Question 5

Indicator in mannitol fermentation test

Answer 5

Phenol red

This test is for SAU. Old sock odor, yellow halo

Question 6

7.5-10% salt used in Mannitol fermentation for SAU acts as:

Answer 6

Inhibitory agent

Question 7

Positive result in dnase test when methyl green is used as an indicator

Answer 7

Clear zone

If toluidene blue = pink zone

Question 8

Substrate used in cephalosporin test for SAU

Answer 8

Nitrocefin
Positive = pink to red

Cephalosporin is a cell wall inhibitor beta lactam. SAU Has beta lactamase.

Question 9

What tests can differentiate staph from micrococcus since both are catalase positive

Answer 9

1. Microccocus can’t grow anaerobically
2. Resistant to lysostaphin and furazolidone
3. Susceptible to bacitracin
4. Ferments glucose, not oxidize

Question 10

Lap test is associated with

Answer 10

Strep

Positive = red

Question 11

Substrate for lap test

Answer 11

Leucine beta napthylamide

Question 12

Reagent for lap test

Answer 12

Cinnamaldehyde

Question 13

Reagent in PYR test

Answer 13

Used to identify strep pyogenes = positive

N-n-dimethylaminocinnamaldehyde

Question 14

M protein is associated with

Protein A is associated with

Answer 14

1. Strep pyogenes -
2. SAU

interfere with phagocytosis

Question 15

Which virulence factor is responsible for the rashes in scarlet fever

Answer 15

Erythrogenic toxin aka pyrogenic toxin

Question 16

Another name for tsst-1

Answer 16

Pyrogenic exotoxins C

Question 17

Another name for optochin

Answer 17

Ethylhydrocuprine Hcl

Question 18

What test is done to speciate neisseria species

Answer 18

Cho utilization

Question 19

Media used in cho utilization test for neisseria. What indicator is used

Answer 19

CTA; phenol red

Question 20

Reagent in cytochrome oxidase test. This is used as what kind of test for neisseria

Answer 20

Tetramethyl p phenyldiamine dihycdrochlroide

Screening test