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MTAP-1-Microbiology > Gram Negative Bacilli - Enterobacteriaceae > Flashcards

Gram Negative Bacilli - Enterobacteriaceae Flashcards

1
Q

Indicator for TSI(pH)

A

Phenol red

H2s indicator is Sodium thiosulfate and ferric ammonium sulfate

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2
Q

Fermentable sugar for CIN, indicator used is

A

Mannitol

Neutral red

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3
Q

Fish eye appearance on emb

A

Enterobacter

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4
Q

Negative color for NLF in HEA

A

Green or colorless with or without black

Positive is yellow with or without black

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5
Q

Selenite broth can be used for

A

Salmonella and shigella

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6
Q

On SSA, what reaction is indicative of the presence of salmonella and shigella

A

Colorless with black center

If h2s (+) = salmonella 
If neg = shigella
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7
Q

Inhibitors in MAC

A

Crystal Violet and bile salts

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8
Q

Tetrathionate broth is used for ID of

A

Salmonella

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9
Q

Positive reaction for onpg, what organisms are positive

A

Yellow

Serratia, citrobacter, s.sonnei, s.Arizonae, y.enterocolitica,hafnium alvei

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10
Q

What test is useful to detect an organism that produces a red pigment (_____)

A

Gelatin hydrolysis = liquefaction
Serratia
Prodigiosin

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11
Q

Incubation settings in the test used to detect serratia

A

Gelatin hydrolysis test

14 days at 35-37C

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12
Q

Special indication when testing for gelatin hydrolysis to avoid false (+) results

A

Remove nutrient gelatin media from incubator daily and ref for 30min . If truly positive, it should liquefy even when put to ref

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13
Q

What substance is detected\produced in the VP test that indicates the organism is positive for that

A

Acetoin or acetylmethyl carbinol

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14
Q

Reagent used in coblentz method. This is for what test

A

40% Koh plus creatine

VP

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15
Q

What Broths may be used when testing for MR

A

Mrvp broth and Clark lubbs

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16
Q

Sim and tryptophan broth maybe used in what imvic test

A

Indole

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17
Q

What can differentiate k. Oxytoca and k.pneumoniae

A

K oxytoca is indole (+)

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18
Q

Indicator for urease test, what is the positive and negative result color

A

Phenol red
(+) pink
(-) yellow

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19
Q

What organisms are slow urease producers

A 
KEYSC
Klebsiella 
Enterobacter
Yersinia
Serratia 
Citrobacter
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20
Q

What organisms are rapid urease producers

A

Ppm + helicobacter

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21
Q

Positive result if tryptophan agar is used in deaminase test

A

Brown

If phenylalanine = green

Negative for both is yellow

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22
Q

Reagent used in deaminase test

A

Ferric chloride (10%)

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23
Q

What tests are useful to differentiate salmonella and shigella

A
  1. Motility (shigella non motile)
  2. Malonate utilization
  3. H2s production
  4. Decarboxylase
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24
Q

Which among salmonella and shigella is H2s positive

A

SPACED

Salmonella, proteus, arizonae , citrobacter, Edwardsiella,

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25
Most sensitive media to use in H2s production test
Sim
26
__% of this sugar is used in moellers broth to detect the ability of the organism to remove Carboxyl group from amino acids
1% glucose
27
Similar to citrate test, what is the positive result and negative in malonate test
(+) blue | (-) green
28
What media is used in motility test
Sim
29
Purple color in decarboxylase test indicates presence of what organism
Salmonella. Note that shigella cannot ferment glucose present in the moellers broth
30
This is a colorless dye that turns into red e is growth of organisms, meaning that they were motile
TTC- triphenyl tetrazolium chloride
31
In the decarboxylase test what organism is the only positive for arginine
Arginine = citrulline Enterobacter cloacae
32
In the decarboxylase test what organism cannot remove putrescine
K. Pneumoniae
33
In the decarboxylase test, the amino acid for lysine is
Cadaverine
34
Amino acid for arginine
Citrulline
35
In the decarboxylase test what organisms cannot remove cadaverine from arginine
E. Cloacae
36
Which organism resemble e.coli
Shigella
37
This organism is characterized by bipolar staining, using —— stain
Yersinia pestis “plague bacilli” Watson stain If not, methylene blue
38
This organism is known for its stalactite appearance
Y. Pestis on broth media
39
On what media does y.pestis exhibit hammered copper appearance
BAP, meaning glistening
40
Agent for mesenteric lymphadenitis
Yersinia pseudotuberculosis
41
Which 2 organisms can tolerate cold, which cannot
Tolerate = y. Enterocolitica and listeria monocytogenes | Cold sensitive = neisseria
42
The agent for pseudoappendicular syndrome has this characteristic appearance in CIN agar
Yersinia enterocolitica, (enterocolitis) | Bulls eye
43
Shiga like e.coli
VTEC, O157:h7, STEC, EHEC
44
What e.coli type is negative in SMAC
O157:h7
45
Agent for community acquired pneumonia
Klebsiella pneumoniae
46
This agent causes necrotizing colitis due to baby milk powder
Enterobacter
47
Salmonella serotype cholesuis may cause what disease
Bacteremia
48
What salmonella serotype causes gastroenteritis
Salmonella typhi
49
What does s.dysenteriae produce that makes it the most severe form of shigellosis
Neurotoxin
50
Duval’s bacilli
S. Sonnei
51
Site of long term s.typhi
Gallbladder
52
Positive for string test
K.pneumoniae
53
Animal pathogen species of salmonella
S. Bongori
54
What organism is known to produce a yellow pigment which intensifies at room temp
Enterobacter
55
Serogroup of new castle Manchester bacilli
S. Boydii | Serogroup C
56
What 3 enzymes , serratia produces
1. DNase 2. Gelatinase 3. Lipase
57
Freshwater inhabitant
Pleisiomonas shigelloides Secretory diarrhea in AIDS
58
Cholera like diarrhea
Etec
59
6th group in diarrhegenic e.coli
DEAC = found in healthy people
60
E.coli serogroup responsible for diarrhea in both child and adult
EAEC
61
E.coli responsible for infantile diarrhea
EPEC
62
Test to characterize e.coli that causes hemolytic uremic syndrome
Mug (4-methylumbelliferyl-b-d-glucoronidase) Negative result o157:h7

MTAP-1-Microbiology (2 decks)

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