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MTAP-1-Microbiology > Gram Negative Bacilli - Enterobacteriaceae > Flashcards

Gram Negative Bacilli - Enterobacteriaceae Flashcards

1
Q

Indicator for TSI(pH)

A

Phenol red

H2s indicator is Sodium thiosulfate and ferric ammonium sulfate

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2
Q

Fermentable sugar for CIN, indicator used is

A

Mannitol

Neutral red

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3
Q

Fish eye appearance on emb

A

Enterobacter

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4
Q

Negative color for NLF in HEA

A

Green or colorless with or without black

Positive is yellow with or without black

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5
Q

Selenite broth can be used for

A

Salmonella and shigella

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6
Q

On SSA, what reaction is indicative of the presence of salmonella and shigella

A

Colorless with black center

If h2s (+) = salmonella 
If neg = shigella
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7
Q

Inhibitors in MAC

A

Crystal Violet and bile salts

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8
Q

Tetrathionate broth is used for ID of

A

Salmonella

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9
Q

Positive reaction for onpg, what organisms are positive

A

Yellow

Serratia, citrobacter, s.sonnei, s.Arizonae, y.enterocolitica,hafnium alvei

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10
Q

What test is useful to detect an organism that produces a red pigment (_____)

A

Gelatin hydrolysis = liquefaction
Serratia
Prodigiosin

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11
Q

Incubation settings in the test used to detect serratia

A

Gelatin hydrolysis test

14 days at 35-37C

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12
Q

Special indication when testing for gelatin hydrolysis to avoid false (+) results

A

Remove nutrient gelatin media from incubator daily and ref for 30min . If truly positive, it should liquefy even when put to ref

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13
Q

What substance is detected\produced in the VP test that indicates the organism is positive for that

A

Acetoin or acetylmethyl carbinol

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14
Q

Reagent used in coblentz method. This is for what test

A

40% Koh plus creatine

VP

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15
Q

What Broths may be used when testing for MR

A

Mrvp broth and Clark lubbs

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16
Q

Sim and tryptophan broth maybe used in what imvic test

A

Indole

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17
Q

What can differentiate k. Oxytoca and k.pneumoniae

A

K oxytoca is indole (+)

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18
Q

Indicator for urease test, what is the positive and negative result color

A

Phenol red
(+) pink
(-) yellow

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19
Q

What organisms are slow urease producers

A 
KEYSC
Klebsiella 
Enterobacter
Yersinia
Serratia 
Citrobacter
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20
Q

What organisms are rapid urease producers

A

Ppm + helicobacter

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21
Q

Positive result if tryptophan agar is used in deaminase test

A

Brown

If phenylalanine = green

Negative for both is yellow

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22
Q

Reagent used in deaminase test

A

Ferric chloride (10%)

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23
Q

What tests are useful to differentiate salmonella and shigella

A
  1. Motility (shigella non motile)
  2. Malonate utilization
  3. H2s production
  4. Decarboxylase
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24
Q

Which among salmonella and shigella is H2s positive

A

SPACED

Salmonella, proteus, arizonae , citrobacter, Edwardsiella,

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25
Q

Most sensitive media to use in H2s production test

26
Q

__% of this sugar is used in moellers broth to detect the ability of the organism to remove Carboxyl group from amino acids

A

1% glucose

27
Q

Similar to citrate test, what is the positive result and negative in malonate test

A

(+) blue

(-) green

28
Q

What media is used in motility test

29
Q

Purple color in decarboxylase test indicates presence of what organism

A

Salmonella. Note that shigella cannot ferment glucose present in the moellers broth

30
Q

This is a colorless dye that turns into red e is growth of organisms, meaning that they were motile

A

TTC- triphenyl tetrazolium chloride

31
Q

In the decarboxylase test what organism is the only positive for arginine

A

Arginine = citrulline

Enterobacter cloacae

32
Q

In the decarboxylase test what organism cannot remove putrescine

A

K. Pneumoniae

33
Q

In the decarboxylase test, the amino acid for lysine is

A

Cadaverine

34
Q

Amino acid for arginine

A

Citrulline

35
Q

In the decarboxylase test what organisms cannot remove cadaverine from arginine

A

E. Cloacae

36
Q

Which organism resemble e.coli

37
Q

This organism is characterized by bipolar staining, using —— stain

A

Yersinia pestis “plague bacilli”
Watson stain
If not, methylene blue

38
Q

This organism is known for its stalactite appearance

A

Y. Pestis on broth media

39
Q

On what media does y.pestis exhibit hammered copper appearance

A

BAP, meaning glistening

40
Q

Agent for mesenteric lymphadenitis

A

Yersinia pseudotuberculosis

41
Q

Which 2 organisms can tolerate cold, which cannot

A

Tolerate = y. Enterocolitica and listeria monocytogenes

Cold sensitive = neisseria

42
Q

The agent for pseudoappendicular syndrome has this characteristic appearance in CIN agar

A

Yersinia enterocolitica, (enterocolitis)

Bulls eye

43
Q

Shiga like e.coli

A

VTEC, O157:h7, STEC, EHEC

44
Q

What e.coli type is negative in SMAC

45
Q

Agent for community acquired pneumonia

A

Klebsiella pneumoniae

46
Q

This agent causes necrotizing colitis due to baby milk powder

A

Enterobacter

47
Q

Salmonella serotype cholesuis may cause what disease

A

Bacteremia

48
Q

What salmonella serotype causes gastroenteritis

A

Salmonella typhi

49
Q

What does s.dysenteriae produce that makes it the most severe form of shigellosis

A

Neurotoxin

50
Q

Duval’s bacilli

51
Q

Site of long term s.typhi

A

Gallbladder

52
Q

Positive for string test

A

K.pneumoniae

53
Q

Animal pathogen species of salmonella

A

S. Bongori

54
Q

What organism is known to produce a yellow pigment which intensifies at room temp

A

Enterobacter

55
Q

Serogroup of new castle Manchester bacilli

A

S. Boydii

Serogroup C

56
Q

What 3 enzymes , serratia produces

A
  1. DNase
  2. Gelatinase
  3. Lipase
57
Q

Freshwater inhabitant

A

Pleisiomonas shigelloides

Secretory diarrhea in AIDS

58
Q

Cholera like diarrhea

59
Q

6th group in diarrhegenic e.coli

A

DEAC = found in healthy people

60
Q

E.coli serogroup responsible for diarrhea in both child and adult

61
Q

E.coli responsible for infantile diarrhea

62
Q

Test to characterize e.coli that causes hemolytic uremic syndrome

A

Mug (4-methylumbelliferyl-b-d-glucoronidase)

Negative result o157:h7

MTAP-1-Microbiology (2 decks)

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