Gram-Positive Bacilli Flashcards
displays Babes-Ernst granules
Corynebacterium sp.
grows on Loeffler’s serum agar
Corynebacterium sp.
CTBA agar
cystine-tellurite blood agar
Corynebacterium sp. shows this on CTBA agar
brown halo
used to measure toxigenicity of C. diphtheriae
Elek’s test
diphtheria toxin: fragment A function
cytotoxic, inhibits protein synthesis
the diphtheria antitoxin is made in ______
horses
diphtheria toxin: fragment B function
binds to cell membrane receptors to mediate entry of fragment A
diphtheria toxin production is inversely proportional to ______________
iron availability
Corynebacterium morphology under microscope
club-shaped
the two forms of diphtheria
respiratory and cutaneous
how diphtheria toxin causes death
toxin enters blood, systemic infection, cardiac failure
Listeria morphology under microscope
coccobacillus
humans are exposed to listeria in ________ (2 things)
dairy products and processed meat
listeriolysin O function
hemolytic and cytotoxic; allows organism to survive inside macrophages
Listeria surface protein p60 function
induces phagocytosis through adhesion and penetration into mammalian cells
effects of listeriosis in pregnant women
premature labor and spontaneous abortion
Listeria grows at this unusual temperature
4 C
Listeria shows this kind of motility at 25 C
umbrella
Listeria shows this kind of motility in a wet prep
tumbling
Listeria is catalase-_______
positive
Corynebacterium is catalase-_______
positive
Listeria CAMP test produces ______-hemolysis
block
Arcanobacterium shows this on agar
black dot under colonies
Arcanobacterium and Erysipelothrix are catalase-_______
negative
Gardernella vaginalis stains gram-_______
variable
grow G. vaginalis on these types of agar (2 types)
human blood bilayer Tween agar (HBT) and V agar
atmospheric requirement for Nocardia
aerobic
Nocardia staining properties
weakly acid-fast, gram-variable
this genus looks like a molar tooth on agar
Actinomadura
Actinomadura colonies are white but can _________
become red
develops salmon-pink pigment after 4-7 days
Rhodococcus
N. cyriaciageorgica and N. farcinica cause
necrosis with abscesses
N. braziliensis causes
actinomycotic mycetomas
Streptomyces smell
woodsy
Streptomyces appearance
waxy, glabrous, heaped
B. anthracis capsule is composed of ________
antiphagocytic D-glutamate
cutaneous anthrax appearance
painless black pustule
pulmonary anthrax symptoms
fever, fatigue, malaise; then respiratory distress, coma, death w/n 24 hrs
four types of anthrax
cutaneous, pulmonary, gastrointestinal, injectional
B. anthracis morphology (agar and microscope)
agar: medusa-head/beaten egg white colonies, string of pearls when exposed to penicillin
microscope: bamboo
B. anthracis - hemolysis and motility
nonhemolytic, nonmotile
B. cereus - hemolysis and motility
beta-hemolytic, motile (flagella)
Bacillus is catalase-______
positive
Bacillus causes these diseases
food poisoning and eye infections
2 types of Bacillus food poisoning
emetic (fried rice) and diarrheal (meat, poultry, soup)
spore-forming gram-positive rods
Bacillus sp.
Erysipelothrix causes ________
painful swelling of hands