Gram-Negative cocci

Question 1

General Neisseria atmospheric requirements

Answer 1

aerobic & capnophilic

Question 2

Most Neisseria are oxidase-_______ and catalase-_______

Answer 2

positive, positive

Question 3

General Neisseria morphology

Answer 3

diplococci attached side-to-side

Question 4

N. elongatata & N. bacilliformis are exceptions because

Answer 4

they are catalase-negative and rod-shaped

Question 5

N. weaver is an exception because

Answer 5

it is catalase-positive but rod-shaped

Question 6

Neisseria are usual flora in these locations

Answer 6

upper respiratory and urogenital tracts

Question 7

this Neisseria species is never normal flora

Answer 7

N. gonorrhoeae

Question 8

the 2 primary Neisseria pathogens

Answer 8

N. gonorrhoeae and N. meningitidis

Question 9

N. gonorrhoeae - major virulence factor

Answer 9

pili w/ antigenic variation

Question 10

N. gonorrhoeae - other virulence factors

Answer 10

capsule; outer membrane proteins w/ antigenic variation; outer membrane porin proteins, lipooligosaccharide endotoxin, IgA protease

Question 11

N. gonorrhoeae outer membrane proteins work by

Answer 11

causing production of useless antibodies

Question 12

N. gonorrhoeae - modes of transmission

Answer 12

sexually or via birth canal

Question 13

__% of men show symptoms of gonorrhoeae; __ - __% of women are _________

Answer 13

95; 20, 80, asymptomatic

Question 14

Gonorrhoea complication in women

Answer 14

pelvic inflammatory disease (PID)

Question 15

disseminated gonococcal bacteremia can lead to

Answer 15

gonococcal arthritis (#1 causative agent in teens and young adults)

Question 16

occurs from infection during vaginal delivery

Answer 16

ophthalmia neonatorum

Question 17

gonorrhoeal infections your newfangled sexual practices will give you

Answer 17

pharyngitis and anorectal infections

Question 18

grow Neisseria on this agar in this atmosphere

Answer 18

modified Thayer-Martin agar in 3 - 5% CO2

Question 19

component of MTM that inhibits gram-positive bacteria

Answer 19

vancomycin

Question 20

component of MTM that inhibits gram-negative bacteria

Answer 20

colistin

Question 21

component of MTM that inhibits yeast

Answer 21

nystatin

Question 22

component of MTM that inhibits swarming Proteus sp.

Answer 22

trimethoprim

Question 23

can you use a gram stain to presumptively identify N. gonorrhoeae?

Answer 23

yes, but only in penile specimens

Question 24

positive oxidase reaction color change

Answer 24

pink to black

Question 25

what is cystine trypticase agar?

Answer 25

agar with 1% of a single carbohydrate and a phenol red indicator

Question 26

N. gonorrhoeae ferments ________

Answer 26

only glucose (sometimes weakly)

Question 27

N. meningitidis ferments __________

Answer 27

glucose and maltose

Question 28

N. lactamica ferments ________

Answer 28

glucose, maltose, lactose

Question 29

N. sicca ferments ________

Answer 29

glucose, maltose, sucrose

Question 30

advantage of nucleic acid assays

Answer 30

can use directly from specimen

Question 31

define superoxol test & N. gonorrhoeae result

Answer 31

30% H2O2, N. gonorrhoeae shows immediate bubbling

Question 32

strain of N. meningitidis that most commonly causes disease in infants

Answer 32

type B

Question 33

some symptoms of meningitis

Answer 33

frontal headache, stiff neck, nausea and vomiting, sometimes fever

Question 34

important clinical infection caused by M. catarrhalis

Answer 34

otitis media

Question 35

tests to ID M. catarrhalis

Answer 35

asacrolytic; DNase positive; reduces nitrate