gram negative bacteria

Question 1

3 forms of disease caused by Legionella pneumophilia

Answer 1

1. Legionnaires’ disease - rapidly progressive disease
2. Pontiac fever - a brief flu-like illness with high attack rate but low mortality
3. non-pneumonic legionnaire’s disease - urine Ag positive but no resp/flu Sx

Question 2

What agar to grow legionella on?

what conditions?

Answer 2

Buffered charcoal yeast extract (BCYE) - with iron and cysteine supplementation

inc at 35-36’C in 2.5-5% CO2

grows opal grew white textured cut-out glass colony in 5-10days