Gram Negative Bacilli Flashcards
Enterobactreiaceae
5 tribes Escherichiae Escherichia Salmonella Shigella Citrobacter
Klebsiellae Klebsiella Hafnium Serratia Enterobacter
Proteae
Proteus
Providentia
Morganella
Erwineae
Erwinia
Yersiniae
Yersinia
Escherichia types
Adherent/pathogenic Invasive Toxigenic Adherent / pathogenic Aggregative Haemorrhagic/vero toxigenic Diffusely adherent to epithelial cells
Toxigenic strains features E. coli
Heat labile strong immunogenicity Heat stable weak immunogenicity CFA TYPES 4 ( colonising Factor antigen) Travellers diarrhoea Non inflammatory diarrhoea
Enteropathogenic E. coli
Adhesion Fimbriae Plasmid encoded Effacement Invades mucosal brush border cells Locus for enterocyte effacement(LEE) Pathogenicity island in chromosome
Non inflammatory diarrhoea
Infants and children M/C
Haemorrhagic strain of e coli
Strain O157:H7 shiga like toxin
Mechanism
Inhibits 23s rRNA ribosome(large subunit)
Inihibit protein synthesis
Lyse vero cells
Aka vero cytotoxin
Cause ARF HUS and Haemorrhagic colitis d/t microangiopathy
Findings
Thrombocytopenia
Non Coombs haemolytic anemia
Inflammatory diarrhoea( eating hamburger,salads )
Invasive E. coli
Inflammatory diarrhoea NON Motile Non lactose fermenter Therefore biochemically atypical Sereny test -KC in rabbit eye Epithelial cytotoxin ( OMP ENDOTOXINS LPS)
Diffusely adherent E. coli
Genes coding surface fimbria
IMVIC TEST
E. coli. \++ - - Klebsiella - - + + Yersinia - - +- Pasteruella multocida \+- - - Vibrio cholera classical O1 - - + -
Oxidase positive organisms
Non enterobacteriaeceae Vibrio cholera fermentation Campylobacter Helicobacter Aeromonas Pastuerella multocida Burholderia pseudomallei Pseudomonas
Fastidious organisms
All except burkholderia malleipp
Aggregative E. coli
Brick pattern in hep 2 cell lines
No serotypes
Shiga toxin
Two types
Shiga type 2 >type 1
M/c in developing countries
Shiga like toxin
Six gene
M/c in developed countries
Fimbriae in E. coli
Mannose resistant fimbriae
P,type 1,f1c,s,m
P uropathogenic E. coli
Upstream strains
Colonising factor antigen
4 types
Down stream strains
Pfeiffers bacilli
Koch weeks bacilli
Freidlanders pneumonia
H influenza
H aegypticus
Multiple abscess formation with severe bronchopneumonia and chronic destructive lesions
Pneumonia causing gram negative BACILLI
oxidase negative Klebsiella pneumonia Yersinia pestis Serratia Acinetobacter baumanii
Oxidase positive
Legionella pneumophila
Pseudomonas
H influenza
Fraciscella Tulare sis
Safety pin appearance
Yersinia Pastuerella Hemophilus ducreyi Calymatobacter granulomatis( dovanosis) vibrio para hemolyticus Burholderia pseudomallei
