Gram Neg Diplococci Exam 1

Question 1

List the organisms in this category.

Answer 1

Neisseria meningitidis, Neisseria gonorrhoeae, and Moraxella catarrhalis

Question 2

All the organisms in this section are oxidase ___

Answer 2

Positive

Question 3

Which organism is not normal flora?

Answer 3

Neisseria gonorrhoeae (STD)

Question 4

What incubation do these organisms prefer?

Answer 4

Increased CO2 levels

Prefer humid conditions (achieved using a candle jar)

Question 5

True/false? Neisseria gonorrhoeae grows well on SBAP

Answer 5

False. Grows on chocolate.

Question 6

Which organisms can grow on SBAP and chocolate?

Answer 6

N. meningitidis and M. catarrhalis

Question 7

What media is selective for N. meningitidis and N. gonorrhoeae?

Answer 7

Modified Thayer-Martin Media

Question 8

What should you do if you suspect N. gonorrhoeae?

Answer 8

Should be plated immediately to media and placed in CO2 environment.

Question 9

What can be used on direct gram stain?

Answer 9

Male urethral discharge suspected of gonorrhea. Females are not diagnostic- normal flora resemble Neisseria spp.

Question 10

When is it ok to presumptively ID N. gonorrhoeae?

Answer 10

Sexually active adults.

Oxidase +, gram - diplococci that grow on selective media for N. gonorrhoeae.

Question 11

What specimen is Moraxella catarrhalis recognized as significant if found in?

Answer 11

Sputum

Question 12

What organism’s colonies can be slid across the plate like a hockey puck?

Answer 12

Moraxella catarrhalis

Question 13

What is Moraxella catarrhalis definitive ID?

Answer 13

Gram Stain, oxidase +, and positive tributyrin hydrolysis test (N. is neg). Another test is DNase Test Agar used to differentiate Moraxella (turns media colorless) and Neisseria spp.

Question 14

What is the treatment for N. gonorrhoeae?

Answer 14

NO longer penicillin

Rocephin in combination with either azithromycin or doxycycline.

Question 15

What is the treatment for N. meningitidis?

Answer 15

Intravenous penicillin may still be used
Broad spectrum antibiotic along with corticosteroids
(can lead to Ab resistance and asthma to infants).

Question 16

True/false? N. gonorrhoeae is the 2nd most common STD behind chlamydia.

Answer 16

True.

Question 17

What is it called when neonates are delivered vaginally by infected mothers with gonorrhea; can cause blindness?

Answer 17

Gonococcal ophthalmia

Question 18

How is meningitis spread?

Answer 18

Via respiratory or throat secretions via kissing
Not spread as easily as easily as the cold or flu
Not spread by breathing the air near infected person

Question 19

What is the 2nd leading cause of community acquired meningitis in the US?

Answer 19

Meningococcal meningitis

Question 20

What is it called when N. meningitidis is in the blood (bacteremia)?

Answer 20

Waterhouse-Friderichsen Syndrome

Question 21

What serotypes of N. meningitidis is most encountered in the US?

Answer 21

Groups A, B, C, Y, and W135