Gram + Cocci Flashcards

0
Q

Catalase -

A

Streptococcus

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1
Q

Catalase +

A

Staphylococci

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2
Q

Gram + cocci

A

Staphylococcus

Streptococcus

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3
Q

Gram +

A

Staphylococcus, Streptococcus, Bacillus, Corynebacterium, Mycobacterium

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4
Q

Coagulase +

A

Staph aureus

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5
Q

Coagulase -

A

S. epidermidis

S. saprophyticus

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6
Q

Ferments mannitol

A

Staphylococcus

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7
Q

Staph transmission

A

Person to person
Airborn
Surgical wounds
Contaminated food: potato salad

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8
Q

Staph aureus virulence factors

A

Polysaccharide capsule
Slime layer
Clumping factor - fibrinogen

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9
Q

Staph aureus virulence immune system defense

A

Protein A
Enzymes: catalase, coagulase, hyaluronidase
Toxins: hemolysis
Transpeptidase

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10
Q

Staph aureus tissue invasion virulence

A

Hyaluronidase
Staphylokinase
Toxins: hemolysin, TSST, enterotoxin, exfoliative toxin

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11
Q

Superantigen

A

Exotoxins

Binds MHC-II

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12
Q

Staph aureus tissue diseases

A
Skin infections
Septic arthritis
Acute endocarditis 
Osteomyelitis 
Meningitis
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13
Q

Staph aureus toxin mediated diseases

A

Gastroenteritis and food poisoning
Scalded skin syndrome
Toxic shock syndrome

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14
Q

Staph aureus treatment

A

Penicillin
Methicillin
Vancomycin - MRSA

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15
Q

Contaminated blood cultures and prosthetics

A

Staph epidermidis

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16
Q

Biofilms, slime layer, prosthetics

A

Staph epidermidis

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17
Q

Staph epidermidis treatment

A

Vancomycin

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18
Q

Leading cause of UTIs in newly sexually active females

A

Staph saprophyticus

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19
Q

Staph saprophyticus treatment

A

Penicillin

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20
Q

Staph agar cultures

A

Sheep blood for hemolytic

Mannitol salt agar

21
Q

Mannitol salt agar

A

Culture for staph

Mannitol fermenting = yellow

22
Q

Staph aureus color on mannitol salt agar

A

Ferments mannitol = yellow

23
Q

Hemolytic patterns on blood agar

A

Alpha - incomplete
Beta - complete
Gamma - none

24
Q

Novobiocin testing

A

Distinguishes saprophyticus from epidermidis

25
Q

Novobiocin sensitive

A

Staph epidermidis

26
Q

Novobiocin resistant

A

Staph saprophyticus

27
Q

Streptococcus species

A

Pyogenes
Pneumonia
Agalactiae
Viridans

28
Q

Streptococcus classification

A

Hemolytic patterns

Lancefield grouping

29
Q

Group A strept

A

Streptococcus pyogenes

30
Q

Group A strept virulence factors

A

Hyaluronic capsule
F protein
C5a peptidase
M protein

31
Q

Group A strept enzymes and toxins

A

Enzymes: streptolysin O, streptolysin S, streptokinase
Toxins: Superantigens

32
Q

Group A strept diseases

A
Pharyngitis
Scarlet fever 
Necrotizing fasciitis 
Toxic shock syndrome
Rheumatic fever
Acute post streptococcal glomerulonephritis
33
Q

Pharyngitis

A

Strept pyogenes
Reddened sore throat, fever, headache
Throat swab
RAPID test

34
Q

Rheumatic fever

A

Untreated streptococcus pharyngitis

Fever, rash, arthritis

35
Q

Necrotizing fasciitis

A

Progresses rapidly and destroys fat and fascia
Systemic shock
Pathogensis from tissue invasion enzymes
Penicillin

36
Q

Strept pyogenes

A

Blood agar: beta hemolytic
Sensitive to bacitracin
PYR test

37
Q

Group B strept

A

Streptococcus agalactiae

38
Q

Virulence of group B strept

A

Polysaccharide capsule

39
Q

Group B diagnosis

A

CAMP reaction
Staph aureus + agalactiae = CAMP factor
Visible hemolysis

40
Q

Diplococci, catalase negative, alpha hemolytic, no lance field antigens

A

Strept pneumonia do

41
Q

Transmission of strept pneumonia

A

Normal flora nasopharynx

Upper respiratory tract infections

42
Q

Strept pneumonia virulence

A

Polysaccharide capsule
IgA protease
Pneumolysin

43
Q

Pneumolysin

A

Cytotoxic creates pores in target cells

44
Q

Rusty sputum

A

Streptococcus pneumonia

45
Q

Pneumonia

A

Bacteria from nasopharynx aspirated deeper

Blood tinged rusty sputum

46
Q

Strept pneumonia infections

A

Otitis media
Sinusitis
Meningitis
Bacteremia

47
Q

Strept pneumonia diagnosis

A
Gram stain
Quellung reaction
Optochin susceptibility 
Bile solubility 
Blood agar: alpha hemolysis
48
Q

Quellung reaction

A

Strept pneumonia diagnosis

Refractiveness around bacteria = +

49
Q

Strept pneumonia treatment

A

Penicillin

50
Q

Viridans strept

A

Alpha hemolytic
Prevalent in oral cavity
Optichin resistant