GPC

Question 1

Micrococcus Disk ID

Answer 1

Bacitracin S
Furazoladone R
Lysostaphin R
Modified oxidase +*
* Can get a false reaction due to waxy nature. Do not perform unless colony appears like staph

Question 2

Staphylococcus DIsk ID

Answer 2

Bacitracin: R
Furazoladone: S
Lysostaphin: S
Mod Oxidase: Neg

Question 3

Rothia

Answer 3

GPC that looks like staph. Nicknamed sticky staph. Encapsulated.
Weak or neg catalase.

Question 4

ID of GBS

Answer 4

Carb of B antigen
CAMP +
Na Hippurate hydrolysis: pos is a purple color

Question 5

ID for GDS

Answer 5

BE and 6.5% Salt.
BE is pos for both Enterococcus and Viridians enterococcus
6.5% Salt is pos only for Enterococcus*
* Aerococcus also grows in 6.5% Salt but is NE negative.

Question 6

GDS common isolates

Answer 6

S. bovis group: Streptococcus gallolyticus, S. bovis, S. equinus, S. infantarius, S. alactolyticus

Question 7

GDS identification from Enterococci

Answer 7

PYR neg while Enterococci are PYR pos
6.5% salt neg while Enterococci are pos
GDS are susceptible to pen while Enterococci are not

Question 8

VP test

Answer 8

Detects product of acetylmethcarbinol or acetoin from glucose metabolism. Pos reaction is a cherry red color.

Question 9

ARG test

Answer 9

Arginine hydrolysis
purple is pos, yellow