GNR Flashcards
Enterobacteriaceae
reactions common
MAC: LF or NLF
Reduces nitrates to nitrites: pos will appear red
* don’t perform oxidase test since it is a waste of time
2 enzymes in LF
1) Permease
2) Beta-galactosidase
ONPG test for
-Tests for beta-galactosidase
-if organism is a late lactose fermenter
PDA Phenylalanine Deaminase
Contains phenylalanine, yeast extract, NaCl
Produces phenyl-lyruvic acid + ammonia
Add FeCl3 -> Green color
MR test
produces strong acids through mixed acid fermentation. A pH indicator turns red when pos
VP test
detects acetoin an end product of the butylene glycol pathway
40% KOH and alpha napthol > red color (pos)
non-motile Enterobacteriaceae
SKY group
Serratia, Klebsiella, Yersinia