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Micro > GNRs > Flashcards

GNRs Flashcards

1
Q

List the four hallmarks of Enterobacteriaceae

A

Nitrate+
Oxidase-
Glucose ferm+
Catalase+

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2
Q

List anaerobic GNRs:

A

Bacteroides
Porphyromonas
Prevotella
Fusobacteria

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3
Q

MacConkey-GROWTH, Glucose-POS, Nitrate-POS, Oxidase-NEG, Catalase-POS

A

Enterobacteriaceae

Proteus
Shigella
Citrobacter
Edwardsiella
Escherichia
Salmonella
Klebsiella
Serratia
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4
Q

MacConkey-GROWTH, Glucose-POS, Oxidase-POS

A

Vibrio
Aeromonas
Plesiomonas

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5
Q

MacConkey-GROWTH, Glucose-NEG

A

Pseudomonas **
Xanthomonas maltophilia
Acinetobacter
Flavobacterium **

*** oxidase-POS

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6
Q

Fastidius organisms

A 
Francisella
Brucella
Bordetella
Pasteurella
Legionella
Campylobacter
Capnocytophaga
Streptobacilus monoliformis
Haemophilis
Helicobacter
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7
Q

List members of the Proteae family:

List a uniformly POS biochemical test in these bitches:

A

Proteus
Morganella
Providencia

**Phenylalanine deaminase

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8
Q

Shigella:

How many organisms does it take to fuck you up?

What lab characteristic is memorable about Shigella?

A

100-200 organisms

**It’s NEG for everything… except METHYL RED

Therefore… lactose? Nope
H2S gas? Nope
VP? Nope
Indole? Citrate? PDA? Nope, nope, nope.

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9
Q

Yersinia entercolitica’s name to fame?

**List three species:

A

MCC of blood transfusion-related sepsis

Remember YCEP*

    • enterocolitica
    • pseudotuberculosis
    • pestis
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10
Q

What other enteric rod does Citrobacter resemble?

How do they differ?

A

Salmonella
*both citrate-POS

Citrobacter is POS in the ONPG reaction (“O-NitroPhenyl-B-D-Galactopyranoside”)

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11
Q

What other enteric rod does Edwardsiella resemble?

How do they differ?

*Any disease associations?

A

Salmonella
**both produce H2S

Edwardsiella is Indole-POS

  • Iron overload
  • co-infection with E. histolytica
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12
Q

Escherichia:

Key BIOCHEM features (2)

A

Lactose-FERM

Indole-POS like Edwardsiella

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13
Q

List types of E. coli infection:

A

ETEC = Enterotoxic = Traveler’s diarrhea
heat-stable vs. guanylate/heat-labile vs. adenylate cyclase

EIEC = Enteroinvasive = Dysentery

EPEC = Enteropathogenic = gastroenteritis in infants of poo’ countries

EAEC = Enteroaggregative = infant diarrhea in poo’ countries

EHEC = Enterhemorrhagic** gets its own flashcard

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14
Q

Which E. coli is O157:H7?

Its biochem characteristic?

A

EHEC

Sorbitol NEG

  • plate on MacConkey-sorbital vs. MacConkey-lactate
  • clear vs. pink = presumptive EHEC!

Miscellaneous shit:
Verotoxin (Shiga-like) = inhibits protein synth and damages enterocytes

Toxin in blood = endothelial damage

HUS

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15
Q

Which Salmonella species cause bacteremia?

MoA (mode of action):

A

S. typhi, paratyphi, and cholerasuis

Invade bowel wall and chill in reticuloendothelial cells of liver, spleen, and gb ==> reinfection

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16
Q

I say Klebsiella, you say… (biochem test)

Disease assoc? (List two)

A

Voges-Proskauer!

Pneumonia in alcoholics (K. pneumniae)
Rhinoscleroma (K. rhinoscleromatis)

17
Q

Which Enterobacteriaceae are non-motile?

A

Klebsiella
Shigella

**Yersinia at 37 C

18
Q

What is prodigiousum?

The microbe that produces this is known for what?

A

A red pigment produced by some strains of Serratia

Nosocomial infections from medical devices

19
Q

What else is H2S-producing? (Besides Salmonella)

A

CEP

Citrobacter
Edwardsiella
Proteus

20
Q

Escherichia’s key BIOCHEM features (2)

A

Lactose-FERM

Indole-POS like Edwardsiella

21
Q

What else is VP-pos? (Besides Klebsiella)

A

SHE

Serratia
Hafnia
Enterobacter

22
Q

Which Enterobacteriaceae ferment lactose?

A

KEE

Klebsiella
Escherichia
Enterobacter

23
Q

Interpret this TSI/KIA:

slant/deep
K/K

A

Not Enterobacteria!

PCEM (pchem?)

Pseudomonas
Campylobacter
Eikenella
Moraxella

24
Q

Interpret this TSI/KIA:

slant/deep
K/A

A

Glucose fermented only

SSPY

Shigella
Serratia
Providencia
Yersinia

25
Q

Interpret this TSI/KIA:

slant/deep
K/A +H2S

A

Glucose fermented only + H2S-producing

SPEC

Salmonella
Proteus
Edwardsiella
Citrobacter

26
Q

Interpret this TSI/KIA:

(slant/deep)
A/A

What if it produces gas too (list 2 bugs)

A

Lactose is their bitch

Escherichia

Klebsiella
Enterobacter

(The KEEs! Remember, these are the three lactose fermenters)

27
Q

Media for Vibrio?

Vibrio cholerae vs. parahemolyticus

A

Thiosulfate citrate bile salt agar

V. cholera: Sucrose+

28
Q

TCBS agar: what’s the differentiating factor?

A

Sucrose

V. cholera will be positive (YELLA)

29
Q

Wtf is El Tor biotype?

A

V. cholera with WEAK string test, has B-hemolysis, and agglutinates on chicken RBCs

30
Q

How is V. cholera classified?

A

(1) O Ags (>150)
(2) Biotype (class vs. El Tor)
(3) Serotype (Ogawa, Inaba, Hikojima)

  • O1 causes most cases
  • non-O1, nobody gives a fuck
  • O139 causes cholera in India
31
Q

What’s the point of sucrose in TSI?

A

To rule out Salmonella/Shigella (neither ferment sucrose)

A/A result rules them out

Micro (4 decks)

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