GN organisms

Question 1

1. GNCB? grey transulent colonies on BA, NLF on MAC (can vary) no haemolysis
   2.OX NEG, non motile, oxidative, spot indole NEG

Answer 1

Acintobacter braumannii

Question 2

1. GN NLF on MAC, B haemolytic
2. Facultative, spot indole pos, OX POS, motile
3. Grow on BAMP plate
   Large pink colonies on Yersinia CIN agar. Similar size and shape on Blood containing media but with haemolysis and grey appearance

Answer 2

Aeromonas hydrophila

Question 3

1. GNB Pale yellow pigment on BA, NG on MAC (for cepacia) no haemolysis. Obligate aerobe
2. OX POS, oxidative, spot indole neg
3. Lime green on MH

Answer 3

Burkholderia cepacia

Question 4

1. GN? Ox POS, microaerophilic and NG on MAC or MH
2. Shiny colonies growing along streak lines

Answer 4

Campylobacter sp.

Question 5

1.GN? no haemolysis, oxidase NEG, motile
2. Spot indole neg, LF on MAC
3. Motile, OX NEG, facultative

Answer 5

Citrobacter freundii

Question 6

1. GN? Non haemolytic, LF on MAC, -medium pink, OX NEG
2. Motile, spot indole neg, facultative

Answer 6

Enterobacter aerogenes

Question 7

1. GNB, non hamolytic, V pink on MAC. Moist wet colonies, opaque centre translucent edge
2. Ox neg, spot indole POS, motile

Answer 7

Escherichia coli

Question 8

1. GNB (v small), NG on BA/MAC, MH, oxidase and catalase variable
2. Isolated from eyes, ears sputum, blood
3. grey flat shiny colonies on CHOC

Answer 8

Haemophilus influenzae

Question 9

1. GNB sticky mucoid, opaque centre translucent edge, LF on MAC non haemolytic
2. OX NEG, spot indole NEG, non motile

Answer 9

Klebsiella pneumoniae

Question 10

1. GNCB shiny colonies on BA, non haemolytic, NG ON MAC! Cat pos, non motile
2. Oxidase POS less than 10sec and spot indole POS
3. sensitive to Pc, cephalosporin, TE

Answer 10

Pasteurella multocida

Question 11

1. NLF on MAC, non haemolytic, spot indole POS

Answer 11

Providencia rettgeri

Question 11

1. Grey growth on MAC, swarms, NLF on MAC no swarming without salt, non haemolytic
2. OX NEG, spot indole NEG

Answer 11

Proteus mirabilis

Question 12

1. Green yellow pigment w metallic sheen irregular shape colonies, NLF on MAC non haemolytic
2. Grape like odour, obligate aerobe, spot indole NEG
3. OX POS, Motile

Answer 12

Pseudomonas aeruginosa

Question 13

1. Non haemolytic, OX NEG, motile, NLF on MAC, Spot indole NEG

Answer 13

Salmonella Typhimurium

Question 14

1. Pigmented red strains, not LF at 24hrs(Looks like it but only LF after 48hrs), non haemolytic, OX NEG
2. Motile, spot indole NEG

Answer 14

Serratia marcescens

Question 15

1. OX NEG, non motile, NLF on MAC, spot indole NEG, non haemolytic

Answer 15

Shigella flexneri

Question 16

1. Hint of a olive green pigment on BA, NLF on MAC, non haemolytic
2. OX NEG, oxidative, spot indole NEG, motile
3. After 48hrs growth on MAC can be lavender and pigment on MH slightly greener

Answer 16

Stenotrophomonas maltophilia

Question 17

1. Strain can have fried egg colonies, NLF on MAC (grows only if there is salt- like TCBS), B haemolytic
2. OX POS, motile, spot indole POS, fermentation

Answer 17

Vibrio alginolyticus

Question 18

1. non haemolytic, OX NEG, motile at less than 30 degrees, non motile at 35
2. Fermentative, spot indole v, NLF on MAC

Answer 18

Yersinia enterocolitica