Glycolysis Flashcards
Reaction 1
In: glucose
Out: Glucose-6-phosphate (G6P)
Products: ATP->ADP
Enzyme: hexokinase glucokinase
Reaction 2
In: Glucose-6-phosphate (G6P)
Out: Fructose-6-phosphate (F6P)
Products: none
Enzyme: Phosphoglucose-isomerase
Reaction 3
In: Fructose-6-phosphate (F6P)
Out: Fructose 1,6 bisphosphate (F1,6BP)
Products: ATP->ADP
Enzyme: Phosphofructokinase
Reaction 4
In: Fructose 1,6 bisphosphate (F1,6BP)
Out: Glyceraldehyde-3-phosphate
Dihydroxyacetone-phosphate (DHAP)
Products: none
Enzymes: Fructose biphosphate aldolaseReaction 5
In: Glyceraldehyde-3-phosphate (GAP)
Out: 2 mols GAP
Products: none
Enzymes: Triose Phosphate isomerase
Reaction 6
In: 2 Glyceraldehyde-3-phosphates
Out: 1,3 Bisphophoglycerate (1,3 BPG)
Products: HPO4 2- + NAD+ –>NADH
Enzymes: Glyceraldehyde-3-dehydrogenase
Reaction 7
In: 1,3 Biphosphoglycerate (1,3 BPG)
Out: 3-phosphoglycerate (3-PG)
Products: ADP–>ATP
Enzymes: Phosphoglycerate Kinase
Reaction 8
In: 3-phosphoglycerate (3PG)
Out: 2-phosphoglycerate (2PG)
Products: none
Enzymes: Phosphoglycerate mutase
Reaction 9
In: 2-phosphoglycerate (2PG)
Out: Phosphoenol pyruvate (PEP)
Products: H2O
Enzyme: Enolase
Reaction 10
In: Phosphoenol pyruvate (PEP)
Out: Pyruvate
Products: ADP–>ATP
Enzyme: Pyruvate Kinase
What step causes 2 reactions of everything
Rxn 5
Regulatory Steps
Rxn 1: G6P inhibits hexokinase glucokinase(competitive inhibitor)
Rxn 3: ATP inhibits phosphofructokinase(allosteric) rate limiting(slowest) step where pace is set
Rxn 10: acetyl CoA inhibits pyruvate kinase and F1,6BP activates pyruvate kinase
Glycolysis
In: Glucose
Out: 2 Pyruvate
2 ATP
2 NADH(goes to ETC to be oxidized)
Glycolysis occurs in:
Cytoplasm of all cells
lactic acid fermentation
Process: Pyruvate–>lactate waste
Products: NADH–>NAD+
Enzyme: LDH
