Citric Acid Cycle

Question 1

Reaction 1

Answer 1

In: Acetyl CoA + Oxaloacetate
Out: Citrate
Product: H2O–>CoASH(coenzyme A)
Enzyme: citrate synthase

Question 2

Reaction 2

Answer 2

In: Citrate
Out: Isocitrate
Product: CoASH -->
               NAD+-->NADH
Enzyme: Aconitase

Question 3

Reaction 3

Answer 3

In: Isocitrate
Out: a(alpha)-ketoglutrate + CO2
Product: NAD+–>NADH
Enzyme: Isocitrate Dehydrogenase

Question 4

Reaction 4

Answer 4

In: alpha-ketoglutrate + CO2
Out: succinyl CoA
Product: CoASH-->
               NAD+-->NADH
Enzyme: alpha-Ketogluterate dehydrogenase

Question 5

Reaction 5

Answer 5

In: succinyl CoA
Out: succinate
Product: GDP +Pi-->GTP-->ATP
                -->CoASH
Enzyme: Succinyl CoA synthetase

Question 6

Reaction 6

Answer 6

In: succinate
Out: fumerate
Product: FAD–> FADH2
Enzyme: succinate dehydrogenase

Question 7

Reaction 7

Answer 7

In: fumerate
Out: malate
Product: H20–>
Enzyme: fumerase

Question 8

Reaction 8

Answer 8

In: malate
Out: oxaloacetate
Product: NAD+–>NADH
Enzyme: Malate dehydrogenase

Question 9

Where

Answer 9

mitochondrial matrix

Question 10

products

Answer 10

2CO2, 3NADH, 1FADH2, 1ATP

Question 11

regulated steps

Answer 11

rxn 1: ATP, NADH, Succinyl CoA inhibit citrate synthase
ADP activates
rxn 3: ATP, NADH inhibit isocitrate dehydrogenase
ADP, Ca2+ activates isocitrate dehydrogenase
rxn 4: NADH, SuccCoA inhibit a-KG dehydrogenase
Ca2+, AMP activate a-KG dehydrogenase