Glycolysis

Question 1

Net ATP from Aerobic Glycolysis using Malate aspartate shuttle

Answer 1

7 ATPs

1 NADH = 2.5 ATPs

4 ATP (substrate level phosphorylation – 2 ATP (investment at hexokinase & PFK-1) + (2NADH x 2.5)

Question 2

Net ATP from Aerobic Glycolysis using Glycerophosphate Shuttle

Answer 2

5 ATPs

1 NADH = 1.5 ATPs

4 ATP (substrate level phosphorylation – 2 ATP (investment at hexokinase & PFK-1) + (2NADH x 1.5)

Question 3

Net ATP from Anaerobic Glycolysis

Answer 3

2 ATPs

Question 4

Most potent activator of PFK-1

Answer 4

Fructose-2,6-biphosphate

product of PFK-2

Question 5

Conversion of Pyruvate to Lactate during Anaerobic glycolysis uses what enzyme?

Answer 5

Lactate dehydrogenase

Question 6

Conversion of Pyruvate to Acetyl CoA in Kreb’s cycle uses what enzyme?

Answer 6

Pyruvate dehydrogenase

Question 7

Conversion of Pyruvate to Oxaloacetate in Gluconeogenesis uses what enzyme?

Answer 7

Pyruvate Carboxylase

Addition of Carbon

Question 8

Most common cause of congenital lactic acidosis

Answer 8

Pyruvate dehydrogenase deficiency

Question 9

Most common enzyme defect in glycolysis

Answer 9

Pyruvate kinase deficiency

Question 10

(3) Irreversible steps of Glycolysis

Answer 10

Step 1. Glucose phosphorylation
Step 3. Fructose-6-Phosphate phosphorylation
Step 10. Pyruvate formation

Question 11

(2) Energy/ATP investment steps

Answer 11

Step 1. Glucose phosphorylation

Step 3. Fructose-6-Phosphate phosphorylation