Glycolysis

Question 0

______ has a high affinity (low Km) for its substrate, glucose,and in the liver and pancreatic B islet cells is saturatedunder all normal conditions and so acts at a constantrate to provide glucose 6-phosphate to meet the cell’sneed.

Answer 0

Hexokinase

Liver and pancreatic B islet cells also contain anisoenzyme of hexokinase, glucokinase, which has a Kmvery much higher than the normal intracellular concen-tration of glucose. The function of glucokinase in theliver is to remove glucose from the blood following ameal, providing glucose 6-phosphate in excess of re-quirements for glycolysis, which will be used for glyco-gen synthesis and lipogenesis.

Question 1

All of the enzymes of glycolysis (Figure 17–2) arefound in the ____

Answer 1

Cytosol

Question 2

Rate limiting reaction in glycolysis

Answer 2

PFK1

Question 3

Enolase is inhibited by___

Answer 3

Fluoride

Question 4

In anaerobic glycolysis, Pyruvate is reducedby the NADH to lactate, the reaction being catalyzedby _____.

Answer 4

lactate dehydrogenase

Question 5

Three irreversible and regulated steps in glycolysis:

Answer 5

These re-actions, catalyzed by hexokinase (and glucokinase),phosphofructokinase, and pyruvate kinase, are themajor sites of regulation of glycolysis

Question 6

Substrate level phosphorylation in glycolysis:

Answer 6

phosphoglycerate kinase

Pyruvate kinase

Question 7

Oxidation of Glucose Yields Up to ___ Mol
of ATP Under Aerobic Conditions But Only
2 Mol When O2 Is Absent

Answer 7

38

Question 8

Inherited aldolase A deficiency and pyruvate kinasedeficiency in erythrocytes cause

Answer 8

hemolytic anemia