Glycogen metabolism in muscle and liver Flashcards
What is glycogen?
Glycogen is the polysaccharide storage form of glucose in the body. It serves as an energy reserve and is stored in granules, predominantly in the liver and muscle.
What is the role of liver glycogen?
Liver glycogen is utilized to maintain plasma glucose levels between meals through the process of glycogenolysis, where glycogen is broken down into glucose.
What is the role of muscle glycogen?
Muscle glycogen is required to sustain muscle contraction during physical activity.
How is glycogen formed?
Glycogen is formed from dietary glucose through the process of glycogenesis.
What is the structure of glycogen?
Glycogen is a highly branched polysaccharide of glucose. It consists of glucose molecules linked by α1→4 glycosidic bonds, with an α1→6 branch occurring every 8-14 glucose residues.
Why is the branching structure of glycogen important?
The branching structure of glycogen is important because it provides a large number of ends where enzymes such as phosphorylase and glycogen synthase can act. This allows for rapid breakdown and re-synthesis of glycogen.
What percentage of the weight of the liver does glycogen constitute in the fed state?
In the fed state, glycogen constitutes approximately 10% of the weight of the liver.
What percentage of the weight of muscle does glycogen constitute in the fed state?
In the fed state, glycogen constitutes approximately 2% of the weight of muscle.
Which tissues store more glycogen, muscle or liver?
More glycogen is stored in muscle compared to the liver.
Why does the liver require de novo synthesis of glycogen via gluconeogenesis?
The liver contains less glycogen than is required to sustain glucose metabolism for 24 hours. Therefore, it requires de novo synthesis of glycogen through the process of gluconeogenesis.
What type of linkages are used to form glycogen?
Glycogen is formed through the use of α-1,4 and α-1,6 linkages of α-D-glucose.
What is the process of glycogen breakdown called?
The process of glycogen breakdown is often referred to as mobilization.
What are the breakdown products of glycogen in the liver and muscle?
During glycogen breakdown, glycogen is converted to glucose-1-phosphate in both the liver and muscle. This glucose-1-phosphate can then be further metabolized to meet the different needs of each tissue.
How does muscle mobilize glycogen?
Muscle mobilizes glycogen to fuel its own energy requirements through glycolysis, which supports muscle contraction.
How does the liver convert glycogen to glucose?
The liver converts glycogen to glucose between meals for export to other tissues. It expresses the enzyme glucose 6-phosphatase, which allows it to convert glucose-6-phosphate back to glucose. Muscle does not express this enzyme.
How do glycogen stores in the liver change throughout the day?
After a meal, glycogen stores in the liver rise in response to an increase in blood glucose. Between meals, glycogen stores in the liver fall as glucose is released from liver glycogen to stabilize the concentration of glucose in the blood. Overnight, glycogen stores in the liver are mobilized to help maintain blood glucose concentration.
What is the relationship between blood glucose and liver glycogen stores?
Throughout the day, the level of liver glycogen stores and blood glucose concentration are closely related. After a meal, liver glycogen stores increase as blood glucose rises. Between meals, liver glycogen stores decrease as glucose is released into the bloodstream to maintain blood glucose levels.
How are the (α1→4) linkages in glycogen broken?
The (α1→4) linkages in glycogen are broken by phosphorolysis, catalyzed by the enzyme glycogen phosphorylase. This process removes single glucose units from the non-reducing ends of glycogen, forming glucose-1-phosphate.
What happens when glycogen phosphorylase reaches an α1→6 branch point?
When glycogen phosphorylase reaches an α1→6 branch point, it stops four residues away from the branch point. At this point, a debranching enzyme transfers a block of three glucose residues from the branch point to the non-reducing end of the glycogen chain.