Glassware Terminology Flashcards
What is tolerance or accuracy?
limits of error, nearness to the true value.
Eg. 5.0ml +- 0.005mL
What is precision or reproducibility?
ability to repeatedly produce the same value.
Eg. if 5mL pipette repeatedly delivers 5.1 mL.
Most glassware is calibrated at which temperature?
20 degrees Celsius
What happens if you take something out of the fridge and immediately measure 100ml in flask TD 20C?
It will end up giving you more liquid because it will expand once warm.
TC and TD?
TC= to contain (to hold stated value) TD= to deliver (delivering to another container)
Is a Mohr and serological pipette calibrated to the tip?
Mohr- NO (comes in 0.1ml-25ml sizes)
Sero- YES
Ultrasonic cleaners?
- used for cleaning small items
- high frequency vibrations cause soil to break free from walls.
Semi-automatic pipette washers?
add water until full then siphoning action occurs, it drains faster than it fills.
Glassware dishwasher has how many cycles and describe them.
six. 1-pre-rinse 2- detergent 3,4,5- hot tap water removes all detergent 6- distilled water rinse
Capacity
volume which glassware can hold or deliver. Eg. if a pipette is marked “1 in 1/100”, it has the capacity of 1mL and the graduations are in 0.01mL divisions.
What happens when you measure a liquid that was hot?
Liquid expands when it is hot, if you measure a hot liquid that once it has cooled to room temp. then you will have a a higher concentration and less volume because it will contract.
What happens when you measure a liquid that was cold?
Liquid contracts when it is cold which means that it will expand and you will have more liquid and it will be less concentrated.