Basic Laboratory Equipment Flashcards
1
Q
What is the formula for calculating RFC?
A
RFC= krn(squared)
K=1.118x10-5
r=radius (cm)
n=speed of rotation of motor (rpm)
2
Q
RFC can be increased by and what will cause a greater effect??
A
Increasing radius or RPM.
RPM will have a greater effect
3
Q
Where is the maximum RCF attained?
A
Bottom of the tube
4
Q
Speed of centrifuge is affected by what?
A
Size of load and resistance
5
Q
What can increase resistance in centrifuges?
A
Air resistance, turbulence and dry/worn bearings
6
Q
What should you do once a tube breaks in the centrifuges?
A
Turn off centrifuge and wait 30 mins. Autoclave(heat), cidex rest, bleach outside tubes rinse / dry
7
Q
What do centrifuges do?
A
Accelerate gravitational separation of substances.
