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MLSC 3008 > GIT > Flashcards

GIT Flashcards

1
Q

a exotoxin that targets cells of the intestinal tract.

A

enterotoxin

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2
Q

alters the metabolic activity of the cells, result in an outpouring of electrolytes and fluid

A

enterotoxin

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3
Q

disrupts the structure of individual intestinal epithelial cells

A

Cytotoxin

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4
Q

secretory and/or absorption activities no longer function, strong host response causes further damage, pus and blood are seen in the stool

A

Cytotoxin

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5
Q

a.k.a. as “enteric fever”, diarrheal disease caused by Salmonella Typhi and S. Paratyphi

A

Typhoid

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6
Q

diarrheal disease caused by Vibrio cholera

A

chlorea

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7
Q

microbial mix of the human bowel

A

90% are anaerobes, remaining facultative anaerobes: Enterobacteriaceae, Enterococcus, yeast, GBS, GGS

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8
Q

survives stomach acid by an increased urease production, results in chronic gastritis and peptic ulcers

A

Helicobacter pylori

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9
Q

Breath test in a Dr.’s office: *C13 or *C14 labelled urea drink, patient exhales *CO2 which is measured is used for

A

Helicobacter pylori

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10
Q

(2) viruses that cause diarrhea

A

Rotavirus, Norwalk virus

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11
Q

three (3) parasites that cause diarrhea

A

Giardia lambia, Cryptosporidium sp, Entamoeba histolytica

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12
Q

Ova & Parasites transportation medium

A

SAF = sodium acetate / acetic acid / formalin
PVA = polyvinyl alcohol
fresh specimens rec.

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13
Q

collection + transport of stool specimens for C&S

A

Carey Blair Transport Media –transported at room temp
Buffered glycerol – better for Shigella
fridge/freeze, fresh is best

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14
Q

collection + transport of stool specimens for viruses

A

clean vial, see BCCDC for transport regulation

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15
Q

this plate screens for Salmonella, Shigella and contains Lactose, sucrose, & salicin, H2S

A

HEK

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16
Q

the plate screens for Yersinia enterocolitic

A

YSA and CIN

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17
Q

differential ingredients of YSA and CIN

A

Mannitol plus bile salts

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18
Q

purpose use of GN broth

A

for Shigella and salmonella

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19
Q

purpose of Selenite broth

A

Recovery of Salmonella and Shigella, inhibits coliform and fecal strep. subculture must be done before 18 hr b/c of breakthrough growth

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20
Q

plates incubated at 42 with Camp pack 48-72 hrs

A

CAMPY, CVA with blood, Charcoal blood free PYL

21
Q

define rice water stools

A

poo that contains flecks of whitish material (mucus and GI epithelial lining)

22
Q

BIO ID of Salmonella

MAC, HEK, MIL, Urea, Citrate, TSI

A

MAC: NLF. HEK: clear green with black centre. MIL: +/-/+ Urea: -ive Citrate: pos. TSI K/A H2S+

23
Q

treatment and AST for uncomplicated gastroenteritis Salmonella

A

self limiting. no abs can prolong duration of convalescent carriage

24
Q

BIO ID of Shigella

MAC, HEK, MIL, Urea, Citrate, TSI

A

MAC: NLF HEK: clear green MIL: -/-/- Urea: neg. Citrate: neg TSA: K/A

25
Q

treatment and AST for Shigella

A

amp, SxT, ciprofloxacin

26
Q

contains deoxycholate and citrate, inhibits gp

A

for recovery of Shigella and Salmonella

27
Q

BIO ID of E. coli

MAC, MIL, Urea, Citrate, TSI

A

MAC:LF +/+/+ urea and citrate: -ive. TSI: A/A gas

28
Q

treatment and AST for E. coli

A

only for paediatric patients

29
Q

treatment and AST for Campy

A

empirical. Erythromycin and fluroquinolones

30
Q

which org appears as bull’s eye clonies on CIN

A

Yersinia

31
Q

BIO ID of Vibrio, Oxidase, glucose, lactose, decarbs, esculin

A

oxidase pos, glucose pos, lactose v, lysine and orthinine pos, arginine neg, esculin neg

32
Q

plate incubates at 22 C/02

A

CIN, YSA

33
Q

main cause f viral gastroenteritis in children

A

rotavirus

34
Q

virus that is from community outbreaks, cruise ships

A

Norwalk virus

35
Q

Thiosulfate Citrate Bile-salts Sucrose Agar detects

A

vibrio species

36
Q

motile at 22 but not 37

A

Yersinia

37
Q

oxidase pos, rice water stools

A

Vibrio spp

38
Q

most common traveler’s diarrhea cause

A

ETEC = enterotoxigenic E. coli

39
Q

media for H. pylori

A

BAP with 7-10% blood, CHOC with blood, urea broth

40
Q

causes enteric fever

A

Salmonella Typhi

41
Q

causes food poisoning

A

Salmonella and campy

42
Q

agents of esophagitis

A

Candidas albicans, HSV type 1 and CMV

43
Q

inflammation of rectum

A

proctitis

44
Q

caused extra intestinal infections, like liver disease, wound infections when occurs in water

A

Aeromonas

45
Q

oxidase pos

A

Vibrio and aermonas

46
Q

how to diff Vibrio and Aermonas based on TCBS agar, growth in salt births and O/129 agent

A

aeromonas will show NG in both and is O.129 resistant

47
Q

how to diff vibrios from plesimonas growth in salt broth

A

for plesiomonas not growth in O and1% growth in 6%

48
Q

how to diff plesimonas from aermonas by CIN

A

plesimonas is mannitol neg

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