GIM 3 - Molecular Genetic Techniques Flashcards
1
Q
what does PCR stands for
A
polymerase chain reaction
2
Q
what is PCR?
A
in vitro synthesis of large amount of DNA by copying from small starting quantities - cloning DNA
3
Q
what is used to define the boundaries of synthesis?
A
oligonucleotides
4
Q
how is the product of PCR be separated?
A
by gel electrophoresis
5
Q
how can you determine the presence of absence of product
A
allele specific PCR
6
Q
what gene defect leads to deafness?
A
connexin 26
7
Q
how does oligonucleotide ligation assay work
A
rely on ligation fails for both allele due to the mis-match
8
Q
in what situations would PCR not work
A
too large eg Fragile X too CG
9
Q
how can you detect unknown mutations?
A
DNA sequencing
10
Q
what is used to stop the PCR
A
dideoxyribonucleotides
