Genomics and NGS analysis

Question 0

Outline the sources of errors that could occur during NGS

Answer 0

• PCR artefacts and duplicates: the artefacts will show up as mismatches
• Sequencing errors: made by the machines
• Mapping errors: when mapping algorithm maps a read to a wrong location, which often occur around repeats or low-complexity regions

Question 1

Define genomics

Answer 1

Discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble and analyse the function and structure of genomes