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Molecular Genetics > Genome Organisation > Flashcards

Genome Organisation Flashcards

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1
Q

What is positive super coiling?

A

DNA overrotated

Helix twists on itself

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2
Q

What are the four possible nitrogenous bases for DNA?

A

Adenine
Thymine
Cytosine
Guanine

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3
Q

What are the four possible nitrogenous bases for DNA?

A

Adenine
Thymine
Cytosine
Guanine

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4
Q

Why are Adenine and Guanine called purines?

A

have double rings

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5
Q

Why are Thymine and Cytosine called pyrimidines?

A

Only one ring

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6
Q

What is the approximate amount of base pairs for each turn in the helix of DNA?

A

10

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7
Q

What does the major groove contain?

A

base pair specific information

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8
Q

What does the minor groove contain?

A

base pair non specific information

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9
Q

What are the two main ways for viral genome packaging?

A

Many viruses assemble their capsids around the viral genomes

form protein capsid shells first and then package their genomes into the procapsids

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10
Q

How does ssRNA helical tobacco mosaic virus package its genome?

A

assemble capsid around genome

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11
Q

How do some dsRNA and dsDNA viruses package their genome?

A

some dsDNA viruses (like herpesviruses) and dsRNA viruses (e.g. φ6 and φ12 bacteriophages) form protein capsid shells first and then package their genomes into the procapsids. These viruses use a packaging motor protein that is driven by the hydrolysis of ATP to condense the nucleic acids into a confined space.

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12
Q

What is the typical bacterial genome like?

A

typically comprised one circular chromosome but often harbored extrachromosomal elements in the form of plasmids or phages

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13
Q

How does the bacterial genome form nucleoids?

A

genome interacts with proteins (nucleoid-associated proteins H-NS and HU) and is tightly negatively supercoiled

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14
Q

What is Topoisomerase?

A

The enzyme responsible for adding and removing turns in the coil

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15
Q

When does positive supercoiling occur?

A

DNA overrotated so it twists on itself

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16
Q

When does negative supercoiling occur?

A

DNA underrotated

twists on itself in the opposite direction

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17
Q

How does topoisomerase I catalyse relaxation of negative supercoils

A

(1) cleavage of one DNA strand;
(2) passage of a segment of DNA through the break, and
(3) resealing the break. No ATP energy required for this reaction.

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18
Q

How does topoisomerase I catalyse relaxation of negative supercoils

A

(1) cleavage of one DNA strand;
(2) passage of a segment of DNA through the break, and
(3) resealing the break. No ATP energy required for this reaction.

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19
Q

What is the Hierarchical organization of the eukaryotic genomic DNA

A

double stranded DNA helix
nucleosome
30nm chromatin fiber

20
Q

What is the subunit of chromosomes?

A

chromatin

21
Q

What is chromatin made of?

A

nucleosome

22
Q

What is euchromatin?

A

undergo condensation and decondensation in the cell cycle

23
Q

What is heterochromatin?

A

remains tightly condensed throughout the cell cycle

24
Q

What is a nucleosomes?

A

The nucleosome is a cylinder with DNA organized into ~1.7 turns around the surface.

Nucleosome looks like beads on a string in the chromatin

Nucleosomes contain an octamer of histones around which DNA winds up

Makes chromatin

25
Q

What is the length of DNA in a nucleosome (in base pairs and histones)?

A

146bp - 260bp

histones

26
Q

In base pairs how long is the core DNA?

A

146bp

27
Q

What is Histone H1 for?

A

H1 is associated with linker DNA and can lie at the point where DNA enters or exits the nucleosome.

28
Q

What Histones is a nucleosome made of?

A

2 x H2A
2 x H2B
2 x H3
2 x H4

29
Q

What does the linker DNA region disfavour?

A

nucleosome formation
by
exhibiting a strong preference for sequences that resisting DNA bending

30
Q

What does the linker DNA region disfavour?

A

nucleosome formation
by
exhibiting a strong preference for sequences that resisting DNA bending

31
Q

What does a Nucleosome core particle (NCP) contain?

A

8 histones and 146-147 bp of DNA wrapped around it in ~1.7 left-handed super helical turns

32
Q

What is the linker histone?

A

H1 variants

H1 interacts with the NCPs and linker DNA to facilitate formation of higher order chromatin structures.

33
Q

What connects linker DNA?

A

NCPs are connected by linker DNA strands and a linker histone H1. H1 interacts with the NCPs and linker DNA to facilitate formation of higher order chromatin structures.

34
Q

What are chromatosomes

A

Chromatosomes are nucleosomes that contain linker histones

35
Q

What is the structural motif of the core histones?

A

consists of two short helices flanking a longer alpha-helix. This is important for the dimerization of histones and it contributes to the “globular core” of the nucleosomes.

36
Q

What is the structural motif of the core histones?

A

consists of two short helices flanking a longer alpha-helix. This is important for the dimerization of histones and it contributes to the “globular core” of the nucleosomes.

37
Q

What do flexible histone tails contain?

A

Flexible histone tails projecting out of nucleosome core contain sites for covalent modifications

38
Q

What do flexible histone tails contain?

A

Flexible histone tails projecting out of nucleosome core contain sites for covalent modifications

39
Q

Core histones have a flexible tail. explain

A

Each core histones have a flexible N-terminal tail and H2A and H2B also have a flexible C-terminal tail. The N- and C-terminal histone tails extend out of the nucleosome core structure and it is free of DNA.

40
Q

How can Histones can be reversibly covalently modified on their tails?

A

methylation, acetylation, phosphorylation, ubiquitylation, sumoylation, ADP-ribosylation

41
Q

What is the histone code?

A

epigenetic marking system using different combinations of histone modification patterns to regulate specific and distinct functional outputs of eukaryotic genomes. Histone code is used as an analogue expression to genetic code.

42
Q

What are the PTM writers?

A

Histone acetyl transferase

Histone methyl transferase

Ubiquitin ligase

43
Q

How does chromatin structure regulates expression?

A

The packaging of DNA into chromatin plays a crucial role in regulating its accessibility for RNA polymerases and transcription factors.

The level of chromatin condensation is dependent on the differentiation state of a cell, ranging from a hyper-dynamic, highly accessible structure in embryonic stem cells to a less accessible more condensed form in senescent cells.

The posttranslational modification of histones can alter the properties of chromatin fibers and is therefore a prime candidate to mediate the stable inheritance of chromatin structures.

44
Q

How does chromatin structure regulates expression?

A

The packaging of DNA into chromatin plays a crucial role in regulating its accessibility for RNA polymerases and transcription factors.

The level of chromatin condensation is dependent on the differentiation state of a cell, ranging from a hyper-dynamic, highly accessible structure in embryonic stem cells to a less accessible more condensed form in senescent cells.

The posttranslational modification of histones can alter the properties of chromatin fibers and is therefore a prime candidate to mediate the stable inheritance of chromatin structures.

45
Q

What are the roles of histone modification?

A

Structural changes of chromatin (regulating chromatin condensation and DNA accessibility):

Replication

Transcription (activation/repression)

Histone deposition on DNA

Recombination

DNA repair

DNA methylation

Molecular Genetics (4 decks)

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