Genome Manipulation 3 Flashcards
1
Q
What is the miRISC complex?
A
A complex formed when various proteins bind to miRNA.
2
Q
What happens if the miRISC complex if exactly complementary to mRNA?
A
Recruits RNases, mRNA degradation, no translation.
3
Q
How does lin-4 in C.elegans block protein synthesis?
A
lin-4 is an miRNA with some complementarity to lin-14, binding to lin-14 when there is a high concentration of lin-4. Binding of lin-4 to lin-14 blocks protein synthesis.
4
Q
How does lin-4 bind to lin-14 in C.elegans?
A
By complementary base pairing to 7 complementary elements in the lin-14 mRNA 3’ UTR.
5
Q
How can miRNAs decrease gene expression?
A
By inhibiting translation or causing direct degradation of the mRNA transcript.
