Genome Content (Lec 16) Flashcards
organization and sequence of genetic information contained within a genome is called what?
structural genomics
approximate locations of genes, relative to the location of other genes, based on the rates of recombination is called what?
genetic maps
what are the limitations of genomic maps?
low resolution or detail; do not correspond to physical distances between genes
Define contig
a set of overlapping fragments that form a continuous stretch of DNA
Define single-nucleotide polymorphisms
site in the genome where individual members of a species differ in a single base pair
The specific set of SNPs and other genetic variants observed on a chromosome is called a what?
haplotype
In regards to structural genomics determining the DNA sequences of entire genomes, the number of copies of DNA sequences (greater than 1000 bp) varies from people to people. This is referred to as?
copy-number variations
In regards to structural genomics determining the DNA sequences of entire genomes, markers associated with DNA sequences that are expressed as RNA are called what?
expressed-sequence tags
In regards to structural genomics determining the DNA sequences of entire genomes, molecular bio + computer science = ?
bioinformatics
This aspect of structural genomics may include deletions or duplications; most contain multiple genes and potentially affect the phenotype; have been found to be associated with psoriasis schizophrenia, autism, and mental retardation
Copy-number variations
This aspect of structural genomics may be obtained by isolating RNA from a cell and subjecting it to reverse transcription, producing a set of cDNA fragments; cDNA fragments are sequenced and the sequence identifies the DNA fragment
Expressed-Sequence tags
How does a cDNA sequence differ from the genomic sequence?
no introns
What is the Ab initio approach of bioinformatics?
scans sequence looking for features usually found within a gene
What is the comparative approach of bioinformatics?
looks for similarity between a new sequence and sequences of known genes
Define metagenomics
sequencing genomes of entire communities of organism- for example, the flora of the human gut
Define synthetic biology
the creation from scratch novel organisms
Define functional genomics
characterizes what the sequences do, The Encode project
All the RNA molecules transcribed from a genome are called what?
transcriptome
All the proteins encoded by the genome are called what?
proteome
Genes that are evolutionary related are called what?
homologous
Homologous genes in different species that evolved from the same gene in a common ancestor are called what?
orthologs
Homologous genes arising by duplication of a single gene in the same organism are called what?
paralogs