Genetics Flashcards

0
Q

What is a triplet?

A

A sequence if 3 bases (codon) that codes for a specific amino acid.

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1
Q

What type of reaction joins nucleotides?

A

Condensation

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2
Q

What is messenger RNA?

A

mRNA is a long strand that is arranged into a single helix
Is a mirror image of the copied DNA strand
mRNA leaves the nucleus through the nuclear pores and associates with the ribosomes
Acts as a template onto which proteins are built
Can be easily broken down

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3
Q

What is ribosomal RNA (rRNA)?

A

Makes up ribosomes along with protein.

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4
Q

What is transfer RNA (tRNA)?

A

Single stranded chain folded into a clover shape
There is a part of the molecule that extends out and allows for amino acids to attach
At the opposite end of the molecule is an “anticodon”
The anticodon will pair with the 3 bases on the mRNA molecule
There are different types of tRNA each with a different “anticodon”

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5
Q

What is the enzyme that breaks up hydrogen bonds between DNA?

A

RNA polymerase

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6
Q

What is a karyotype?

A

Total chromosome complement in a typical body cell of a eukaryote.

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7
Q

Where does translation happen?

A

In the cytoplasm on the rough endoplasmic reticulum.

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8
Q

What is a polysome?

A

A group of ribosomes all moving along the same piece of mRNA and translating it simultaneously.

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9
Q

What is a mutation and what are the types of point mutation?

A

A random change in the amount or composition of DNA. Can be either a deletion, insertion or base substitution.

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10
Q

What is a frame shift mutation?

A

Happened when a base is inserted or deleted. All amino acids after this point will then be different, this can cause a non-functioning protein.

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11
Q

What does it mean that DNA is degenerate?

A

There are more codons than amino acids, therefore some amino acids have more than one codon.

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12
Q

What is an anticodon?

A

The triplet of bases at one end of a tRNA molecule that is complimentary to a particular codon on a mRNA molecule.

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13
Q

What is a cistron?

A

A gene

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14
Q

What bonds join amino acids in translation?

A

Peptide bonds

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15
Q

What is a transcription factor?

A

A protein molecule that controls the transcription of genes

16
Q

What do siRNA do?

A

Inhibit gene expression at translation

17
Q

What does RNA polymerase do?

A

Joins nucleotides together to form mRNA/premRNA/RNA.

18
Q

What is a promoter?

A

A specific region just upstream of a gene that acts as a binding site for transcription factors and RNA polymerase during the initiation of transcription.

19
Q

What is the difference between pre-mRNA and mRNA?

A

pre-mRNA contains introns.

20
Q

Why are the percentage of bases from the middle and end parts of the chromosome different?

A

They will have different genes. Different combinations of bases. Pre-mRNA transcribed from different DNA/ codes for different proteins.

21
Q

What causes siRNA to attach to only one sort of mRNA?

A

Has complementary base sequence.

22
Q

Suggest why siRNA may be useful in treating disease.

A
  1. Some diseases are genetic / caused by mutations;
  2. siRNA will stop product of this gene / the protein being produced / stops translation.
23
Q

Give two features of stem cells.

A

Will replace themselves/ replicate;

Undifferentiated/can differentiate/develop into other cells/totipotent/multipotent/pluripotent;

24
Q

Why does oestrogen only affect target cells?

A

Other cells don’t have oestrogen receptors.