Genetic Technology

Question 1

Gel electrophoresis:

Answer 1

• Seperate different m.s in analysis of proteins and DNA.

- Placing mix of agarose gel and applying an electrical field.

Question 2

Movement of charges m.s in gel depends on 3 factors:

Answer 2

1. Net (overall) charge: neg. charged move to anode (+), pos. to cathode (-). Highly charged m.s move faster.
2. Size: smaller m.a move faster through gel.
3. Composition of gel: Size of “pores” in gel determines speed which proteins and fragments of DNA move.

Question 3

Applications of genetic engineering:

Answer 3

• Production of human insulin.
• GM crops.
• Pest resistance.
• Herbicide resistance.