Genetic technology

Question 1

What is meant by recombinant DNA technology?

Answer 1

DNA combined from different sources using enzyme to create GMO.

Question 2

Describe and explain how the level of expression of some genes can be identified using DNA microarray analysis technique.

Answer 2

Use mRNA to make sscDNA

Fluorescence tag attached to CDNA.

Hybridise cDNA with probe.

Use UV light to show fluorescence.

Intensity of flurescence indicates the level of gene expression.

Question 3

Give two advantages of genetically engineered herbicide resistance crop plants.

Answer 3

Reduce cost.
Greater crop yield.

Question 4

Describe the advantage of databases.

Answer 4

Large amount of DNA sequences is stored.

Able to compare nucleotide / amino acids sequence.

Easily accessible and shared by the scientific community.

Question 5

Outline the procedure used for gene therapy treatment of a person with SCID.

Answer 5

Remove stem cells.

Mix stem cells with viral vector containing the healthy alleles.

Stem cells injected into blood.

Lymphocytes produce functional ADA.