Genetic engineering stages Flashcards
1
Q
definition of genetic engineering
A
Transfer of DNA from one organism to another .
2
Q
Stage 1
A
restriction enzymes used to isolate required gene , leaving it with sticky ends .
3
Q
stage 2
A
A vector , usually a bacterial plasmid or virus is cut by the restriction enzymes .
leaving it with corresponding sticky ends .
4
Q
stage 3
A
vector and isolated gene are joined together by ligase enzymes
5
Q
stage 4
A
Vector inserts gene into required cells
6
Q
stage 5
A
The genes formed are usually transferred into animal or plant in early stages of development .
7
Q
Define sticky ends
A
Short section of unpaired bases
8
Q
Define vector
A
way in which genetic material is transferred
