Genetic engineering

Question 1

Definition of Inbred

Answer 1

Strain established by a minimum of 20 consecutivegenerations of sister × brother matings (sib matings)

Question 2

Definition of Outbred

Answer 2

Stock/line established and maintained by strictavoidance of sib mating

Question 3

F1 hybrid

Answer 3

Created by mating mice of two inbred strains in adefined direction

Question 4

F2 hybrid

Answer 4

Created by mating two F1 hybrid mice

Question 5

Recombinantinbred

Answer 5

Lines established by inbreeding of the progeny ofindividual F2 hybrid sibling pairs for at least 20generations

Question 6

Congenic

Answer 6

Introduction of a mutation onto a defined inbredstrain background by 5–10 or more generations ofmatings back to the inbred strain with selection forthe mutation (backcrossing)

Question 7

Consomic

Answer 7

An inbred strain that carries one chromosome fromanother inbred strainTen generations of backcrossing and selection for thedonor chromosome is required for a fully consomicstrain

Question 8

Conplastic

Answer 8

An inbred strain that carries the nuclear genome ofone inbred strain and the cytoplasm and cytoplasmic(mitochondrial) genome of anotherSince the egg contributes the embryonic cytoplasm,10 generations of backcrossing inbred females to aninbred male recipient is required for a fully conplasticstrain

Question 9

RFLP

Answer 9

Restriction fragment length polymorphism - DNA is isolated, digested by bacterial restriction enzymes, then the lengths of the digested fragments compared for differences.

Question 10

SNPs

Answer 10

Single Nucleotide Polymorphisms - identified variations in single nucleotides used to monitor genotypes