genetic engineering Flashcards

1
Q

what does cre do

A

catalyze recombination between 2 loxp sites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what is cre

A

dna recombinase (enzyme)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

three types of genetically engineered mice

A

knockout
knock in
conditional knockout

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

why do they make gen ed mice

A

to study gene function and model human diseases bc their genomes are similar to ours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

neo r

A

neomycin resistant gene

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

postitive selection

A

kills the cells without the neor bc neor is positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

negative selection

A

kills the gene with the tk bc its negative and keeps the healthy cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

tk

A

thymidne kinase found in herpes - suicide cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

knockout mice steps

A

-add neor and tk into target gene to make targeting vector sequence
-add the targeting vector into es cells via electroporation
-only cells that have successfully incorporated survive when neo and ganciclovir is added
-cells containing the targeting vector are added to embryo
-results in a chimeric mouse (spotty)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

electroporation

A

creates electricity/pores for dna to get in - like distracting and slipping in through memebrane

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

es cells

A

embryonic stem cells (come from embryos)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

conditional knockout

A

deletion of gene in selected cell types

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

crispr

A

clustered regularly interspaced short palindromic repeats - part of the bacterial immune system

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

cas9

A

enzyme- a becterial endonuclease that cuts dna at a specific site within a larger target sites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

nuclease

A

an enzyme that cleaves (cuts) the bonds of the backbone of nucleic acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

pam

A

downstream of the protospacer sequence based on where the pam is depends on where the cleavage will be

17
Q

guiding region

A

20 nucleotides long and is complementary to the target dna sequence that defines where cas9 cuts

18
Q

scaffold region

A

a region that forms a hairpin loop that binds in a crevice of cas9

19
Q

target dna

A

the sequence of dna that will be cut by cas9

20
Q

sgRNA

A

includes guiding region and the scaffolding region

21
Q

floxed

A

cre will look for the loxp and pull out the stuff in between it