Genetic Engineering Flashcards
The use of molecular techniques to modify the traits of a target organism
Genetic Engineering
Which is considered as traditional way which practices the mating of organisms with desirable qualities
Classical Breeding
A modern technique of genetic engineering. Joining together of DNA molecule is inserted into a host organism to produce new genetic combinations from two different species
Recombinant DNA technology (rDNA)
The general outline of recombinant DNA are as follows:
1)Cutting
2) Selection
3) Ligation
4) Transfer
5) Selection process
6) Sequencing of the gene
Cleavage of DNA
Cutting
Molecular scissors
Restriction enzymes
An appropriate vector or vehicle which would propagate the recombinant DNA
Selection
They are not part of the main cellular genome but they carry genomes or genes that provide the host cell with useful properties
Plasmids
Joining together of the gene interest with the vector
Ligation
What is the name of resulting molecule from ligation?
Recombinant DNA
The recombinant plasmid into a host cell (that would carry out replications to make huge copies of the recombined plasmid)
Transfer
Screen which cells actually contain the gene of interest
Selection process
Find out the primary structure of the protein
Sequencing of the gene
These plasmids or gene copies will now be introduced to its _______
Host organisms
