Genetic Code and Translation Flashcards
What is the order of transcription, translation, and mRNA degradation in prokaryotes
they are simultaneous occurrences
In eukaryotes where do transcription and translation occur
transcription - nucleus
translation - cytoplasm
Why does mRNA last longer in eukaryotes
in prokaryotes all steps occur in the same location simultaneously, in eukaryotes transcription and translation occur in different parts of the cell so mRNA exists for longer in eukaryotes
What researchers led to the “one gene one collinear protein” conclusion
Beadle and Tatum
What bond holds amino acids together
peptide bond (carboxyl of one bonded to amino of the other)
What code does the genetic code follow
triplet code
4^3 = 64 possible codons
How were codons discovered
homopolymers and copolymers were tested and were found to produce polypeptides with different amino acids
How many codons are there
64
How many of the 64 codons are stop codons
3
What does it mean when saying the genetic code is degenerate
some amino acids are specified by more than one codon
What 2 amino acids are specified by only one codon
methionine (start) and tryptophan
Why does degeneracy occur in the genetic code
the binding at the third codon position is flexible
- known as the wobble position
How does wobble occur in translation (think tRNA modification)
since tRNA can be modified at the nucleotide level, nucleotides can be substituted for other nucleotides and create wobble
What is the basics of the wobble hypothesis
nonstandard pairings can occur at the third position of a codon
What is a nickname for the ribosome
RNA machine - plays a role in protein synthesis, including the formation of peptide bonds between amino acids
What are the 4 important macromolecules of translation
- ribosomes
- amino acid activating enzymes
- tRNA molecules
- soluble proteins involved in initiation, elongation and termination
What is the function of aminoacyl tRNA synthetase
charges the tRNA molecule with its specific amino acid
What is the 2 step reaction between the amino acid and tRNA via aminoacyl tRNA synthetase
- amino acid reacts with ATP to our aminoacyl AMP
- amino acid is transferred to tRNA and AMP is released
How to tRNAs work
hold the anticodon for the codon of mRNA
What is another term for translation
protein synthesis
What is required to initiate translation
mRNA, large and small ribosomal subunits, initiation factors, and GTP
What is the initiation complex of translation in prokaryotes
Shine Dalgarno sequence
Where is the complement to the Shine dalgarno sequence held
in the 16S component of the 30S small ribosomal subunit
What is the Shine Dalgarno sequence
UAAGGAGGU
How does the small subunit bind first to prokaryotic mRNA in translation, without the binding of the large subunit
IF-3 binds to the large unit, preventing it from binding, causing only the small subunit to bind (has complementary sequence to Shine Dalgarno)
What positions fMet-tRNA over the start codon
IF-1 and IF-2
What makes up the 30S initiation complex
fMet-tRNA + 30S small subunit + IF2-GTP + IF-1 + IF-3
How does the large subunit then attach to the mRNA in prokaryotic translation initiation
IF-3 dissociates
How are the IF-2 and IF-1 factors dissociated from the initiation complex in prokaryotic translation
GTP is hydrolized into GDP and dissociates the remaining IF factors
What is the final complex at the start of prokaryotic translation called
the 70S complex (think of the overall subunits and complex of prokaryotic ribosomes)