Genes Flashcards
Features of the genetic code
Degenerate - different triplets can code for the same amino acid
Non-overlapping - each base in the sequence is only part of one triplet and is only read once
Universal - each triplet codes for the same amino acid in all organisms
What are introns and exons?
Introns are non-coding parts of DNA.
Exons are coding parts of DNA.
DNA in prokaryotic and eukaryotic cells
Prokaryotic cells have DNA in the form of a single circular loop of free floating DNA. Some also have small rings of DNA called plasmids.
Eukaryotic cells have DNA folded around proteins called histones. The DNA is supercoiled and form structures called chromosomes.
Chromosome structure
Chromosomes are made up of coiled up DNA and histones and they are only visible as distinct structures when a cell is dividing. Chromosomes appear as two threads, called chromatids, joined at a single point, the centromere.
Structure of mRNA and tRNA
mRNA is a long, single strand of nucleotides.
tRNA is made up of around 80 nucleotides in a clover-like shape. The ‘clover leaves’ have unpaired bases sticking out of them, and one of the leaves is the anticodon loop.
Transcription
DNA helicase unwinds part of the DNA
Free RNA nucleotides pair up with the exposed DNA
RNA polymerase joins the RNA nucleotides together to form pre-mRNA
The DNA re-forms and the pre-mRNA leaves the nucleus from a pore in the nuclear envelope
The pre-mRNA is spliced, removing introns so that it becomes mRNA
Translation
After leaving the nucleus, mRNA attaches to a ribosome
Free tRNA molecules with the right anticodon, and their amino acid, bind with the complementary codon on the mRNA
Peptide bonds form between the amino acids and the tRNA is released.
The second and third step repeats until a stop codon is reached
Base mutations
Substitution - where one nucleotide is swapped for another. Usually has a small or no impact due to the degenerate nature of the genetic code.
Deletion - where one nucleotide is lost from the sequence. Can have a huge impact due to the non-overlapping nature of the genetic code.
Chromosome mutations
Individual homologous pairs of chromosomes can fail to separate in meiosis, resulting in a gamete having one more or one less chromosome. This is known as non-disjunction.
Meiosis
During meiosis 1, the first division, homologous chromosomes line up and twist around each other. Equivalent portions of each break off and recombine with the other chromatid; this is known as crossing over (and recombination) and is important for genetic diversity as it creates unique combinations of alleles.
In meiosis 1, the separation of homologous chromosomes into daughter cells is random; this is known as the independent segregation of homologous chromosomes and is important for genetic diversity as it creates genetically different daughter cells.
Calculating chromosome combinations
An organism can produce 2ⁿ possible different combinations of chromosomes as a result of meiosis.
Two organisms can produce (2ⁿ)² possible different combinations of chromosomes in offspring as a result of sexual reproduction.
Types of selection
Directional selection - phenotypes at one extreme of the population being selected for and those at the other extreme being selected against.
Stabilising selection - phenotypes around the mean of the population being selected for and those at the extremes being selected against.
Definition of a species
Members of a species can produce fertile offspring.
Phylogenetic classification
Phylogenetic classification is a form of classification that classifies species into groups based on evolutionary relationships and shared features between organisms and their ancestors. The groups are arranged into a non-overlapping hierarchy.
Taxonomic hierarchy
Domain Kingdom Phylum Class Order Family Genus Species