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Honours Exam LTC > General Protein Stuff > Flashcards

General Protein Stuff Flashcards

0
Q

Work flow of ELISA

A 
Coating Ab at bottom of well
Add sample Ag
Detection Ab (enzyme linked)
Colour substrate 
Read OD
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1
Q

How to generate polyclonal Ab

A

Inoculate mouse
Spin down blood
Isolate serum which contains Abs (IgG)

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2
Q

Dif between ELISA and ELISPOT

A

ELISPOT - Place whole cells on coating Ab, then wash cells then add detection Ab. Spots can be counted

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Honours Exam LTC (10 decks)

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