Gene Expression Flashcards
Mutations in lac Operon
I-
prevents repressor from binding
recessive) (on all the time
constitutive Mutation
prevents binding of repressor to operator
Mutations in lac operon:
Oc
prevents repressor from binding
dominant) (on all the time
mutations in lac operon
Is Superrepressor
repressor stays bound to operon all the time
dominant) (on all the time
Mutations in lac operon:
Z-
Non-functional galactosidase
doesn’t affect expression, only products
Compact DNA (chromatin)
Does not allow transcription to happen
Constitutive Expression
Continuous transcription
no regulation, always being expressed
products needed in large quantities
CRP
cAMP Receptor Protein
CAP
catabolic activator protein
helps activate activator protein but need cAMP to activate
trp Operon
synthesized amino acid, tryptophan
- negative repressible control, repressed by presence or tryptophan
- structural genes encode enzymes of the tryptophan synthesis pathway
- products synthesis substrate (tyrptophan) tryptophan turns off operon