Gen Microbiology Flashcards
These microorganisms thrive at 25-45 degree C temperature
Mesophiles
Psychrotophs are microorganisms that grow at temperature between ____
20 to 30 degree C
TRUE or FALSE: Psychrotophs grow well at low temperature
TRUE
identify the nutrition of the microorganism
Photoautotroph
energy source and carbon source
sunlight CO2
identify nutrition of microorganism
Photoheterotrophs
nutrition in the form of energy source and carbon source
sunlight and organic compounds
identify the nutrition of microorganism
Chemoautotrophs need ____ and ____ for growth
**inorganic compounds and CO2 **
Chemoheterotrophs need ____ for growth
organic compounds
Acidophiles function best at what pH?
below 5.5
Neutrophiles work best at what pH?
pH between 5-8
Alkaliphiles work at a pH of ____.
pH of above 8.5
Give the two kinds of aerobes
- strict/obligate aerobe
- microaerophiles
This aerobe needs mandatory oxygen
Obligate aerobe
They are capable of growing with lower levels of O2
Microaerophiles
Enumerate the kinds of anaerobes
- obligate anaerobe
- facultative anaerobe
- aerotolerant anaerobe
give a characteristic
What is a facultative anaerobe?
Facultative anaerobe can grow with or without O2
give a characteristic
What is an obligate anaerobe?
Obligate anaerobes die in the presence of O2
This anaerobe can protect itself from reactive oxygen molecules
Aerotolerant anaerobe
What are aerotolerant anaerobes?
These anaerobes use fermentation to produce ATP. They can also withstand reactive oxygen molecules
He invented the first compound microscope
Zaccharias Janssen
He is the first person to observe organisms using the microscope
Robert Hooke
Compare Abiogenesis Theory and Biogenesis Theory
Abiogenesis theory states that life emerges from nonliving matter while for Biogenesis Theory, it provides that life can only come from other lifeforms
Biogenesis Theory - Life begets life
What is the Germ Theory of Disease?
The theory was discovered by Louis Pasteur and it explains that diseases originated from pathogens or from disease-causing microorganisms (e.g. Staphylococcus, streptococcus)
What was Robert Koch’s contribution?
He cultivated anthrax bacteria (Bacillus anthacis) and proved that bacteria plays a major role in diseases, demonstrating it by injecting pure cultures of the bacilli in mice and seeing that it caused anthrax.
Invented Koch’s Postulates
Utilized vaccines to prevent diseases like cholera, anthrax, and wine erysipelas
Louis Pasteur
______ developed a second type of ______ vaccine, which was live-attenuated (using the virus in weakened form)
Albert Sabin, polio
Salvarsan, the first antimicrobial agent and a remedy for syphilis, was synthesized by _______.
Paul Ehrlich
_______ discovered ________ or also known as “miracle drug”
Alexander Fleming, penicillin
Give requirements for growth of halophiles
They need 10-15% salt
Microorganisms that thrive at high salt concentrations higher than those necessary for their growth.
Halotolerant
Kinds of eukaryotes
Fungi, algae, protozoa
Kinds of prokaryotes
Bacteria, archaea
Three domains of microorganisms
Archaea, bacteria, eukarya
List the five major phyla of fungi
- Chyrtridiomata
- Zygomycota
- Ascomycota
- Basidiomycota
- Glomeromycota
Six major groups of algae
- Chlorophyta (green algae)
- Phaeophyta (brown algae)
- Rhodophyta (red algae)
- Pyrrophyta (dinoflagellates,fire algae)
- Euglenophyta (euglenoids)
- Chrysophyta (golden brown algae and diatoms)
5 major groups of protozoa
- Mastigophora
- Sarcodina
- Ciliophora
- Sporozoa
- Microspora
A set of rules and guidelines used for naming bacteria, archaea, and other prokaryotic microorganisms
International Code of Nomenclature of Prokaryotes (ICNP)
Give the taxonomy order for organisms
Domain
Kingdom
Phylum
Class
Order
Family
Genus
Species
What are the major characteristics used in taxonomy
- Morphological
- Biochemical
- Ecological
- Physiological
- Pathogenicity
- Genetic
- Antigenic
Algaes can cause diseases via toxins. List the kinds of toxins
- Saxitoxin (paralytic shellfish poisoning)
- Brevetoxin (neurotoxic shellfish poisoning)
- Ciguatoxin (ciguatera fish poisoning)
- Dinophysistoxin (diarrhetic shellfish poisoning)
- Domoic acid (amnesic shellfish poisoning)
____ is the sum of all chemical reactions within a living organism
Metabolism
identify what species
Is known as brewer’s yeast
Saccharomyces cerevisiae
Causes infections in humans (candidiasis). It is a pathogenic yeast
Candida albicans
identify what species of yeast
Used in ferementation and winemaking (beneficial microbe)
Saccharomyces bayanus
identify what spp. of algae
Is a seaweed used to produce nori (sushi wrap)
Pyropia yezoensis
identify what algae sp.
Used in preparation of food supplements
Chlorella vulgaris
what algae sp.
Is a source of algin, a binding and emulsifying agent used in production of foods, cosmetics
think of antonym sa micro, and remember peri peri
Macrocystic pyrifera
Is the most investigated microalgal species; spirulina
Arthrospira platensis
identify sp. of protist
causes tricomoniasis
Trichomonas vaginalis
causes giardiasis
Giardia duodenalis
This protist causes malaria
Plasmodium falciparum
aids in carbon cycle by feeding on decaying plants and animals
Paramecium
is a facultative anaerobe, causes diarrheal illness in humans through consumption of contaminated foods of animal origin (e.g. eggs, poultry, fish, milk and dairy products, etc.)
Salmonella
name two bacteria species
Are acid-tolerant and beneficial organisms used in fermentation and as probiotics
Lactobacillus d. bulgaricus & Streptococcus thermophilus
Some strains of this bacteria are pathogenic; indicator of fecal contamination or unsanitary processing
E. coli
The only Listeria sp. that is associated with human listeriosis, which is contracted through post-process recontaminatoin of final product
Listeria monocytogenes
Are unicellular, extremophiles, and have unique cell wall and cell membrane
Archaea
Tolerates extreme salinity
Halobacterium salinarum
Plays a role in methane production
Methanosarcina barkeri
Oxidizes sulfur to produce sulfuric acid, responsible for HSO4 concentration in aquatic environments
Sulfolobus acidocaldarius
List the 5 steps of viral replication
- Attachment (phase attaches to surface of host cell)
- Penetration (viral DNA enters host cell)
- Biosynthesis (phage replicates to make phage proteins)
- Maturation (new phage particles are made)
- Lysis (cell lyses and newly made phages are released)
A technique where inoculum is added to pre-solidified agar
Streak plate technique
A technique where sample is pipetted first before adding the sterile agar/broth
Pour plate technique
A technique used for detecting low levels of microorganisms in liquid samples; uses sterile membrane filter with a pore size that retains the microbes
Membrane filter technique
This allows for the** characterization of individual microbial cells** with unique properties and functions
Single-cell isolation technique
A culture preservation method that is achieved by first freezing the culture at very low temperature
Freeze-drying/lyophilization
Method where specimens are frozen along with a cryoprotective agent (glycerol) in liquid-nitrogen refrigerators
Freezing with liquid nitrogen
2 types of microscope
- Light microscope
- Electron Microscope
List the kinds of light microscope and briefly describe them
- Bright field - uses bright background, objects are darker
- Dark field - uses darker background, objects are bright or luminous
- UV - uses shorter wavelengths of light
- Fluorescence - uses fluorochromes
- Phase contrast - used for detailed examination of the internal structure
Microscope used to examine viruses and ultrastructure in thin sections of cells
Transmission electron microscope (TEM)
Microscope used to observe surface features of cells and viruses
Scanning electron microscope (SEM)
Give the 4 methods of controlling microbial growth that use moist heat as an agent
- Boiling
- Pasteurization
- Autoclaving
- Tyndallization
Give the 2 methods of controlling microbial growth that use dry heat as an agent
- Direct flame (incineration)
- Hot air
Using low temperature as agent, what are the 2 methods to control microbial growth
- Refrigeration by storage at 4 C
- Deep freezing at 0 to -95 C
Give 2 methods to control microbial growth using desiccation as agent
- Drying by sun, air, oven
- Freeze-drying
Filtration uses methods such as:
1.
2.
- Heat sensitive materials
- filtration of air using HEPA
__ and __ rays are radiation with high energy that causes atoms to lose electrons which can result to DNA destruction. Are excellent ionizing and sterilizing agents that penetrate deep into the object
X-rays and gamma rays
Method that uses non-ionizing radiation to control microbial growth. Does not penetrate objects leading to surface sterilization only
UV radiation (absorbed readily by the DNA to cause damage)
Is a halogen that is used often as a topical tincture
Iodine
identify the chemical agent to control microbial growth
Is iodine complexed with an organic compound to increase iondine’s stability
Iodophor
identify chemical agent
- ____ is used on burns
- ____ acts as an algicide
- ____ disinfects skin mucous membrane
these are heavy metals
- silver sulfadiazine
- copper sulfate
- merthiolate
identify the chemical agent
This gas is highly penetrating and can sterilize heat-sensitive items like catheters, syringes, etc.
Ethylene oxide
what chemical agent
Is used as a standard for the effectiveness of other disinfectants
Phenol
____ are phenolics used to control microbial growth
Cresols
Is a broad-spectrum disinfectant and biocide which kills viruses, bacteria, fungi, endospores
Formalin (37% formaldehyde)
identify the chemical agent
These are used as skin antiseptics
is an oxidizing agent
H2O2
identify contributor
Developed a type of filtration known today as the Chamberland filter. Also credited for their research project leading to the invention of the autoclave
Charles Chamberland
Are microorganisms that thrive at 70 to 110 C
Hyperthermophiles
One difference between bacteria and eukarya, and archaea is that the former have an ____ linkage while archaea has an ____ linkage
ester, ether
The products of light-dependent reactions are (which are also needed in the calvin cycle:
1.
2.
3.
- ATP
- NADPH
- O2
Involves the fixation of carbon dioxide into organic compounds like glucose which is catalyzed by enzyme RuBisCO
Calvin cycle
List the 4 methods of genetic transfer
- Conjugation (cell-to-cell contact)
- Transduction (transfer of bacterial genes by viruses)
- Transformation (bacteria cells take up free DNA and use it for their own genome)
- Protoplast Fusion (fusion of genetic material of 2 or more bacterial cells)
Two types of Transduction:
1.
2.
1. Generalized transduction
2. Specialized transduction (transfer of specific region of the host chromosome)
Is the killing, inhibition or removal of microorganisms which may cause diseases
Disinfection
Is the reduction of microorganisms to safe levels according to public standards
Sanitation
Is the prevention of infection or sepsis in living tissues using chemicals
Antisepsis