Gen Charac Of Bacteria Flashcards
True or false:
Prokaryotic and eukaryotic cells both contain nucleic acids, proteins, lipids, and carbohydrates.
True
True or false:
Prokaryotic and Eukaryotic cells use the same kinds of chemical reactions to metabolize food, build proteins, and store ener
True
True or false:
Prokaryotic and eukaryotic cells have the same structure of cell walls and membranes, and the absence of organelles.
False. They differ in structure of cell walls and membranes, and the absence of organelles .
CHIEF DISTINGUISHING CHARACTERISTICS OF
EUKARYOTES:
- DNA is found in the _____________, which is separated from the cytoplasm by a nuclear membrane, and the DNA is found in multiple chromosomes
- DNA is consistently associated with chromosomal proteins called ___________ and with nonhistone
- Have a number of ____________________
- Cell walls, when present, are chemically __________
- Cell division usually involves __________
- DNA is found in the cell ‘s nucleus, which is separated from the cytoplasm by a nuclear membrane, and the DNA is found in multiple chromosomes
- DNA is consistently associated with chromosomal proteins called histones and with nonhistone
- Have a number of membrane-enclosed organelles
- Cell walls, when present, are chemically simple
- Cell division usually involves Mitosis
CHIEF DISTINGUISHING CHARACTERISTICS OF
PROKARYOTES
- DNA is not enclosed within a membrane and is usually a __________________ chromosome (prokaryotes have no true nucleus)
- DNA is not associated with _____________; other proteins are associated with the DNA.
- Lack _________________
(ex: mitochondria, golgi apparatus) - Cell walls almost always contain the ____________________
- Usually divide by ____________
→DNA is copied, and the cell splits into two cells
→involves fewer structures and processes than eukaryotic cell division
- DNA is not enclosed within a membrane and is usually a singular circularly arranged chromosome (prokaryotes have no true nucleus)
- DNA is not associated with histones; other proteins are associated with the DNA.
- Lack membrane-enclosed organelles
(ex: mitochondria, golgi apparatus) - Cell walls almost always contain the complex polysaccharide peptidoglycan
- Usually divide by Binary Fission
→DNA is copied, and the cell splits into two cells
→involves fewer structures and processes than eukaryotic cell division
- unicellular organisms that lack a nuclear membrane and true nucleus
- classified as prokaryotes (Greek: before kernel [nucleus]), having no mitochondria, endoplasmic reticulum (ER), or Golgi bodies (they only have ribosomes)
Bacteria
Vary in size, morphology, and cell to-cell arrangements and in the chemical composition and structure of the cell wall
BACTERIAL MORPHOLOGY
Bacterial cell wall differences provide the basis for the __________
Gram stain
Most clinically relevant bacterial species range in size from ___________ in width and ___________ in length
Most clinically relevant bacterial species range in size from 0 .25 to 1 μm in width and 1 to 3 μm in length
True or false:
Bacterium is some hundred-fold larger than a virus, and ten-fold smaller than a eukaryotic cell
True
True or false:
Variation of size and shape within a population may also result from asymmetric growth of the cell wall
True
Bacterial shape
Cocci
Coccobacilli
Bacillus
Fusiform
Curved
Spiral
Pleomorphic
Cluster: ______________
Chains: ______________
Pairs: __________
Tetrads:__________
Cluster: Staphylococci
Chains: Streptococci
Pairs: Diplococci
Tetrads:Micrococcus
BACTERIAL ARRANGEMENT
a) Pairs
b) Chains
c) Grape-like clusters
d) Group of four
e) Packets of eight
f) Palisades
g) Chinese characters
outermost structure
comprises of outer membrane, cell well, periplasm, cytoplasmic or cell membrane
Cell envelop
- Found only in gram-negative bacteria
- Function as cell’s initial barrier to the environment
Outer membrane
a void space between your outer membrane and inner membrane
periplasmic space
- Bilayered structure composed of lipopolysaccharide Which gives the surface of gram-negative bacteria a net negative charge
- Plays a significant role in the ability of certain bacteria to cause disease
Outer membrane
- Protein structures scattered throughout the lipopolysaccharide macromolecules
- Water-filled structures that control the passage of nutrients and other solutes, including antibiotics,
through the outer membrane - Number and types of porins vary with bacterial species
- Influence the extent to which various substances pass through the outer membranes of different bacteria
Porins
Facilitate the attachment of the outer membrane to
the next internal layer in the cell envelope, the cell
wall
MUREIN LIPOPROTEINS
- Referred to as the PEPTIDOGLYCAN, or MUREIN
LAYER - Gives the bacterial cell shape and strength to
withstand changes in environmental osmotic
pressures that would otherwise result in cell lysis
CELL WALL ( MUREIN LAYER)
- Protects against mechanical disruption of the cell and offers some barrier to the passage of larger
substances - Synthesis and structure are often the primary targets for the development and design of several
antimicrobial agents
Cell wall (murein layer)
Cell wall structure is composed of __________________
DISACCHARIDE-PENTAPEPTIDE SUBUNITS
- Alternating sugar components (moieties),with the
amino acid chain linked to N-acetylmuramic acid molecules - Polymers of these subunits cross-link to one another by means of peptide bridges to form peptidoglycan
sheets - Layers of these sheets are cross-linked with one
another, forming a multilayered, cross-linked structure of considerable strength
N-ACETYL-D-GLUCOSAMINE AND N-ACETYL-D-MURAMIC ACID
Referred to as the_____________, or sack, this
peptidoglycan structure surrounds the entire cell
MUREIN SACCULUS
Major types of cell walls:
gram-positive and gram-negative types
stain gram-positive, have a modified cell wall called ACID-FAST CELL WALL
Mycobacteria
microorganisms that have no cell wall
Mycoplasmas
Primary stain use in acid-fast stain
carbol fuchsin
- Composed of a very thick protective peptidoglycan (murein)layer
- Consists of glycan (polysaccharide) chains of
alternating N-acetyl-d- glucosamine (NAG) and N-
acetyl-d-muramic acid (NAM) - many antibiotics effective against gram-positive
organisms (e.g., penicillin) act by preventing synthesis
of peptidoglycan
GRAM-POSITIVE CELL WALL
thinner layer of peptidoglycan
and a different cell wall structure, are less affected by these antibiotics
Gram-negative bacteria
OTHER COMPONENTS OF GRAM-POSITIVE CELL WALL THAT PENETRATE TO THE EXTERIOR OF THE CELL
TEICHOIC ACID
LIPOTEICHOIC ACID
TEICHURONIC ACID
- anchored to the peptidoglycan (N-acetylmuramic acid)
- glycerol or ribitol phosphate polymers combined with
various sugars, amino acids, and amino sugars
TEICHOIC ACID
- anchored to the PM (plasma membrane)
- linked to the next underlying layer, PM or cellular
memebrane
LIPOTEICHOIC ACID
two components that are unique to the gram-positive cell wall
TEICHOIC ACID
LIPOTEICHOIC ACID
- similar polymers, but the repeat units include sugar
acids (eg, N-acetylmannosuronic or d-glucosuronic
acid) instead of phosphoric acids - synthesized in place of teichoic acids when phosphate is limiting
TEICHURONIC ACID
Two layers of gram-negative cell wall
Inner peptidoglycan layer
Outer membrane
- much thinner than in gram-positive cell walls
Inner peptidoglycan layer
- Outside the peptidoglycan layer is an additional outer membrane
- contains proteins, phospholipids, and lipopolysaccharide (LPS)
Outer membrane
LPS THREE REGIONS
O-specific polysaccharide
Core polysaccharide
Lipid A (also called endotoxin)
LPS region that is antigenic
O-specific polysaccharide
LPS region with ketodeoxyoctanoic acid (KDO) and heptose
Core polysaccharide
LPS region:
- inner, major constituents
Lipid A (also called endotoxin)
LPS functions:
- Vital in evading the host defenses
- Contribute to the negative charge of the bacterial surface, which stabilizes the membrane structure
- Considered as an endotoxin
- consists of phosphorylated glucosamine disaccharide units to which are attached a number of long-chain fatty acids
- responsible for producing fever and shock conditions in patients infected with gram-negative bacteria
Lipid A moiety
Outer membrane function:
- Acts as a barrier to hydrophobic compounds and
harmful substances - Acts as a sieve, allowing water-soluble molecules to
enter through protein-lined channels called porins - Provides attachment sites that enhance attachment to host cells
- Strong negative charge is an important factor in
evading phagocytosis - Acts as a barriers to toxic substances that prevents movement inside the cell
Peptidoglycan layer of Gram(+) bacteria
Thick(multilayered)
Teichoic Acids of Gram(+) bacteria
Present in many
Periplasmic Space of Gram(+) bacteria
Absent
Outer Membrane of gram (+) bacteria
Absent
LPS content of gram(+) bacteria
Virtually none
Lipid and LPP of gram(+) bacteria
Low
Flagellar structure of gram(+) bacteria
2 Rings in basal body
Toxins produced by gram(+) bacteria
Exotoxins
Resistance to Physical Disruption of gram(+) bacteria
High
Cell Wall Disruption by Lysozyme of gram(+) bacteria
High
Susceptibility to Pen and Sulfonamide of gram(+) bacteria
High
Susceptibility to Strep, Chloram and Tetra of gram(+) bacteria
Low
Inhibitions by Basic Dyes of gram(+) bacteria
High
Resistance to Anionic Detergents of gram(+) bacteria
Low
Resistance to Sodium Azide of gram(+) bacteria
High
Resistance to Drying of gram(+) bacteria
High
Peptidoglycan layer of gram(-) bacteria
Thin (bilayered/trilayered)
Teichoic Acids of gram (-) bacteria
Absent