Gelatin Flashcards
What is gelatin
It is a mixture of RGFP that are derived from animal tissues, particularly skin and bones from particularly pig and cow with hot water
Cartilage bones and skin are generally made up of
Collagen
Gelatin formation process involves the conversion of
Insoluble collagen to soluble gelatin
The starting material and the initial process involved in the preparation of gelatin is
Bones that are defatted with organic solvent and decalcified with acid treatment
What are the types of gelatin
Type A gelatin from partial acid hydrolysis
Type B gelatin from partial alkaline hydrolysis of collagen
Characteristics of gelatin
The form is in strips or granular powder
It is colorless or pale yellow
It has a little odour
It does not dissolve in cold water but absorbs most of the moisture
Long term boiling causes the gelatinizing powder to be reduced
Jelly strength or bloom strength
It is the measure of the quantity of gelatin that can be evaluated using a bloom geometer
Differences between type A and B gelatin
Based on preparation, isoelectric points and compatibility with anionic substances
Chemical constituents of gelatin
Mainly protein glutin because of this, test for protein is positive
What are some of the chemical tests for gelatin
Gelatin is for protein, nitrogen and aromatic amino acids
Protein in general protein test and when it is reacted with mercuric nitrate solution to give a white precipitate turning red on warming
Nitrogen when reacted with soda lime to evolve ammonia
Aromatic amino acids when it is reacted with trinitrophenol solution to give a precipitate
Some uses of gelatin
3P&S
To make pastes, pastilles, pessaries, pill coatings and suppositories
What type of gelatin is used for intravenous injections
Specially purified pyrogen free gelatin
A grade of gelatin with high bloom strength is
Gelatin capsules and bacteriological culture media
What are the characteristics of gelatin sponge
It is water insoluble
It is absorbable
It is a hemostatic material
How is gelatin sponge made
It is made by soaking a piece of foam with uniform porosity in a warm solution of gelatin and drying it, it is then cut into pieces and sterilized with dry heat
What is the difference between gelatin and agar
In the test for nitrogen using soda lime as the reagent, gelatin evolves ammonia unlike agar
