Gel Immunodiffusion & Stan’s Salad Saga

Question 1

precipitation occurs between what?

Answer 1

soluble antigens and homologous antibodies

Question 2

Gel immunodiffusion involves two forms in a saline agar plate. What are they?

Answer 2

Antiserum and unknown antigen composition

Question 3

What is antiserum?

Answer 3

Antibody in the serum is placed to one well and antigen is placed in the well

Question 3

What is antiserum?

Answer 3

Antibody in the serum is placed to one well and antigen is placed in the well

Question 4

if the sample has an antigen that will react to the antibody, what will happen?

Answer 4

a precipitation line will form at the region of optimal portions as their diffusion path pass

Question 5

If no precipitation line worm then what happens?

Answer 5

then it’s negative

Question 6

Gel immunodiffusion can be used as what?

Answer 6

used to identify the presence of antigens and used to check for identical, related, or unrelated antigens based on their epitope

Question 7

What is shown on the plate if the serum has a specific antibody that recognizes the soluble antigen?

Answer 7

White precipitin line

Question 8

Is the test considered positive or negative if the serum has no antibody that reacts with the known antigen?

Answer 8

Negative

Question 9

Precipitation is caused by
the antigen/antibody response

Answer 9

the antigen/antibody response

Question 10

What is the purpose of stan’s pasta saga?

Answer 10

Determine whether stan’s pasta in stan’s salad was the source of the egg that caused his allergic reaction.

Question 11

How might plating with antibiotics disc help you treat Stan if he does develop an infection

Answer 11

It indicates what kind of antibiotic we can use if Stan ever gets an infection

Question 12

How many wells did we have?

Answer 12

1,2,3,4,5,6