Elisa Test: ELISA Immuno Explorer Kit Flashcards

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1
Q

What does ELIZA?

A

Enzyme,Linked ,Immunoabsorbent, Assay

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2
Q

What is ELISA used for?

A

Used to detect antigen or antibody in a sample

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3
Q

What is antigen?

A

Molecules that cause your body to mount an immune response may include components of infectious agents like bacteria, viruses, and fungi

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4
Q

What is Primary Antibodies?

A

antibodies used to recognize antigens like disease agents and confer specificity to the assay.

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5
Q

What is secondary Antibodies?

A

secondary antibodies recognize and bind to the primary antibodies, which are antibodies from another species.

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6
Q

Examples of ELISA In-Clinic Tests

A

heartworm
parvovirus
FeLV, FIV
Lyme, Ehrlichia,
Anaplasma
Giardia
canine brucellosis
progesterone
pregnancy

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7
Q

The purified antigen homologous to the antibody being tested for is added to the wells of the microstripes. True or False

A

True

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8
Q

why is the ELISA test performed?

A

the test is often used to see if you have been exposed to viruses or other substances that cause infection.

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9
Q

What is a blocking agent used for?

A

used to coat the well where antigens have not attached

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10
Q

What is the first step in the indicator reactions

A

adding secondary antibodies to the wells

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11
Q

What is the second step in the indicator reaction?

A

to use the enzyme on the secondary antibody to indicate its presences

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