gel electrophoresis Flashcards
1
Q
explain the first stage of gel electrophoresis
A
DNA fragments put into gel strips that contain buffer solution that maintains constant pH
2
Q
explain the second stage of gel electrophoresis
A
electric current is passed through electrophoresis plate
- DNA fragments in wells (negative end) move through gel towards positive end
3
Q
what does the rate of movement depend on?
A
mass / length of DNA fragments
4
Q
what do the mesh-like structures of the gel do?
A
make it easier for smaller fragments to move through (than larger ones)
5
Q
why does DNA move towards the positive anode?
A
DNA has negatively charged phosphate groups (opposites attract)
