Gel Electrophoresis Flashcards
What is gel electrophoresis?
A technique used to separate DNA fragments based on size and charge.
Importance and use of electrophoresis
Used in DNA profiling, paternity testing, forensics, and research.
Reagents/materials used
Gel, buffer solution, DNA samples, DNA ladder/markers.
Reagents/materials and their functions (Gel)
Gel: Provides a medium for DNA separation.
Reagents/materials and their functions (Buffer solution)
Buffer solution: Conducts electricity and maintains pH.
Reagents/materials and their functions (DNA samples)
DNA samples: Material to be separated.
Reagents/materials and their functions (DNA ladder/markers)
DNA ladder/markers: For size comparison.
Mechanism of electrophoresis
DNA fragments are loaded into wells in the gel and subjected to an electric field, causing them to migrate based on size and charge.
Positioning of wells
Wells are usually at one end of the gel.
Micropipette used
Micropipette: A precision tool used to transfer small volumes of liquid accurately.
Proper usage of a micropipette
Calibrate the micropipette, set the desired volume, aspirate the liquid, dispense accurately, and handle with care to avoid contamination.
