Gel Electrophoresis Flashcards
1
Q
Which of the three plasmid forms migrates the fastest through gel?
A
Supercoiled
2
Q
Which of the three plasmid forms migrates the slowest through gel?
A
Relaxed/nicked
3
Q
Which of the three plasmid forms migrates at an in-between speed through gel?
A
Linearised
4
Q
How can linearised plasmids occur?
A
Either deliberately using a restriction endonuclease, or accidentally during the extraction process
