GCSE 1.2-1.7 and 2.1-2.6 Flashcards
Describe all parts of a light microscope
Ocular lens Body tube Revolving nosepiece Low, medium and high power objectives Arm Stage clips Stage Diaphragm Lightsource Coarse adjustment knob (big) Fine adjustment knob (small) Base
What magnification does the eyepiece lens give
X10
What magnification does each objective lens give and what is the total
Low - X4 so total = X40
Medium - X10 so total = X100
High - X40 so total = X400
What is the purpose of iodine and methylene blue on your temporary mounts (onion slide)
To stain the cells/structures and make them visible under light
Why must the onion tissue be 1 cell thick? What happens if you have more than one layer?
If it is more than one cell thick it will be too hard to see an individual cell as the light can’t reach the top layer
Why is it important to only use the fine focus when viewing at high power?
As it may resulting in crashing into the slide if coarse is used
What happens to the area of the specimen you can see as you increase magnification
You see it closer and therefore more detail
What are the seven process which are common to all living things
Movement Respiration Sensitivity Growth Reproduction Excretion Nutrition
What is something made of a large number of cells
Multicellular
What are organism described as when only made up of one cell
Unicellular
What does a nucleus do
Contains all the chemical reactions that take place inside the cell
Contains all the genetic information needed to produce a new living organism. It is bound by a nuclear membrane
What do chromosomes do
Found within nucleus
Made of DNA
Consist of many genes which control the organisms characteristics
What is a mitochondria
Free in Cytoplasm
The site of cell respiration
The more energy required by a cell, the more mitochondria will be present within it eg muscle and sperm cells
What is cytoplasm
A jelly like substance with hundreds of chemicals in it
Lots of chemical reactions take place in it
What is a cell membrane
A think skin around the cell, holding the cell together
Provides a barrier, and controls what passes in and out of the cell
As only some substances can cross this membrane it is described as selectively permeable
What is a cell wall
Lies outside of cytoplasm
Made of cellulose
Provides a ridged support and so gives the plant cell a particular shape
Name all the parts in a plant cell
Cytoplasm Cell wall Cell membrane (2 lines) Mitochondria Vacuole Chloroplasts Nucleus Nuclear membrane
Name all the parts in an animal cell
Cytoplasm Mitochondria Cell membrane Nucleus Nuclear membrane
What is a large permeable vacuole
Fluid filled space surrounded by a membrane
Contains cell sap, made up of water, minerals and dissolved substances
Keeps the shape of the cell, when full, and keeps the cells rigid giving the plants more support
What are chloroplasts
These are present in plant cells which photosynthesise
Contain a green substance called chlorophyll that trap light energy and help the plant make its own food by photosynthesis
Name the parts of a bacterial cell
Cell wall Cytoplasm Bacterial DNA (chromosomal DNA) Plasmid DNA Flagellum
Give the light microscope equation
Total magnification = magnification of objective lens x magnification of eyepiece lens
What is the magnification triangle
O
M A
O = observed size A = actual size M = magnification
A nucleus in a photograph, magnification X400, measures 15mm. What is its actual size in um
A=O/M = 15/400 = 37.5 um