GCSE 1.2-1.7 and 2.1-2.6 Flashcards
Describe all parts of a light microscope
Ocular lens Body tube Revolving nosepiece Low, medium and high power objectives Arm Stage clips Stage Diaphragm Lightsource Coarse adjustment knob (big) Fine adjustment knob (small) Base
What magnification does the eyepiece lens give
X10
What magnification does each objective lens give and what is the total
Low - X4 so total = X40
Medium - X10 so total = X100
High - X40 so total = X400
What is the purpose of iodine and methylene blue on your temporary mounts (onion slide)
To stain the cells/structures and make them visible under light
Why must the onion tissue be 1 cell thick? What happens if you have more than one layer?
If it is more than one cell thick it will be too hard to see an individual cell as the light can’t reach the top layer
Why is it important to only use the fine focus when viewing at high power?
As it may resulting in crashing into the slide if coarse is used
What happens to the area of the specimen you can see as you increase magnification
You see it closer and therefore more detail
What are the seven process which are common to all living things
Movement Respiration Sensitivity Growth Reproduction Excretion Nutrition
What is something made of a large number of cells
Multicellular
What are organism described as when only made up of one cell
Unicellular
What does a nucleus do
Contains all the chemical reactions that take place inside the cell
Contains all the genetic information needed to produce a new living organism. It is bound by a nuclear membrane
What do chromosomes do
Found within nucleus
Made of DNA
Consist of many genes which control the organisms characteristics
What is a mitochondria
Free in Cytoplasm
The site of cell respiration
The more energy required by a cell, the more mitochondria will be present within it eg muscle and sperm cells
What is cytoplasm
A jelly like substance with hundreds of chemicals in it
Lots of chemical reactions take place in it
What is a cell membrane
A think skin around the cell, holding the cell together
Provides a barrier, and controls what passes in and out of the cell
As only some substances can cross this membrane it is described as selectively permeable
What is a cell wall
Lies outside of cytoplasm
Made of cellulose
Provides a ridged support and so gives the plant cell a particular shape
Name all the parts in a plant cell
Cytoplasm Cell wall Cell membrane (2 lines) Mitochondria Vacuole Chloroplasts Nucleus Nuclear membrane
Name all the parts in an animal cell
Cytoplasm Mitochondria Cell membrane Nucleus Nuclear membrane
What is a large permeable vacuole
Fluid filled space surrounded by a membrane
Contains cell sap, made up of water, minerals and dissolved substances
Keeps the shape of the cell, when full, and keeps the cells rigid giving the plants more support
What are chloroplasts
These are present in plant cells which photosynthesise
Contain a green substance called chlorophyll that trap light energy and help the plant make its own food by photosynthesis
Name the parts of a bacterial cell
Cell wall Cytoplasm Bacterial DNA (chromosomal DNA) Plasmid DNA Flagellum
Give the light microscope equation
Total magnification = magnification of objective lens x magnification of eyepiece lens
What is the magnification triangle
O
M A
O = observed size A = actual size M = magnification
A nucleus in a photograph, magnification X400, measures 15mm. What is its actual size in um
A=O/M = 15/400 = 37.5 um
How much is 1000 um in mm
1mm
How much is 1000mm in m
1m
How much is 1m in um
1,000,000um
If a cell measures 1.3mm what is its length in um
1300
If a cell measures 27.5um what is its length in mm
0.0275mm
Give some facts about electron microscopes
Use an electron beam instead of light, which is focussed using electromagnets
Specimen has to be specially prepared and held inside vacuum chamber from which the air has been pumped out
Image is formed as photograph (electron micrograph)
Very large pieces of equipment that require special rooms in a laboratory
Resolution of a light microscope
0.20um
Resolution of an electron microscope
0.25x10 to the power of -3um
Magnification using light microscope
Up to X1500
Magnification using electron microscope
Up to X500,000
Give some specialised animal cells
Sperm cell
Ciliates epithelial cells
Nerve cells
Give some specialised plant cells
Root hair cells
Onion epidermical cells
Plant leaf palisade mesophyll cells
Cells to organism flow chart
Cells -> tissue -> organ -> organ system -> organism
What forms a tissue
Group of specialised cells working together
What forms an organ
Tissues working together
What forms an organ system
Organs working together
What forms an organism
Organ systems working together
Define diffusion
The random movement of molecules from an area of high concentration to an area of low concentration, down a concentration gradient
What is net movement
Overall movement
Give an example of diffusion of gases
Oxygen required for respiration passes through gas exchange surfaces eg alveoli by diffusion. The carbon dioxide produced by respiration diffuses in the opposite direction
Why is smoke visible coming out of a chimney but becomes invisible very quickly
It disperses in air. High concentration of soot particles which diffuse into surrounding air becoming dispersed and appearing invisible
What are stem cells
Cells which are undifferentiated and have not yet become specialised
What are the 2 important abilities stem cells have
To continue dividing by cell division to produce more stem cells
To differentiate into a wide variety of specialised cells
Where can stem cells be harvested from
Umbilical comes of babies immediate after birth
Placenta of babies immediately after birth
Early human embryos that are not used in fertility treatments
Give an example of stem cells being used in medicine
Bone marrow transplants in treating Leukaemia
Digestive organs
Mouth, stomach, small + large intestines
Respiratory organs
Trachea, bronchi, lungs
Skeletal organs
Ribs and bones
Circulatory organs
Heart + blood vessels
Excretory organs
Liver, kidneys, + bladder
Nervous organs
Brain + spinal cord
Reproductive organs
Testes + ovaries
Main organs in plants
The root
The stem
The leaves
The flower
Describe where stem cells occur in plants
In the meristems of plants
Word equation for photosynthesis
Carbon dioxide + water -light energy-chlorophyll-> glucose + oxygen
Balanced chemical equation for photosynthesis
6CO(2) + 6H(2)O -light energy-chlorophyll-> G(6)H(12)O(6) + 6O(2)
Describe the parts of a leaf
Very top - wax cuticle Top chunk - upper epidermis Middle chunk - mesophyll Upper middle - palisade mesophyll Lower middle - spongy mesophyll Bottom chunk - lower epidermis Gap - stoma Bits before gap - guard cell
Give some adaptations of the leaf for light absorption
Thin leaves Learned surface area Cuticle Upper epidermis Palisade mesophyll layer Chloroplasts
What are endothermic reactions
reactions which require or absorbs energy from its surrounding, usually in the form of heat
What is a better way of measuring the rate of photosynthesis than counting bubbles
Bubbles could be different sizes so should measure volume of oxygen produced
What happens in the light intensity graph of rate of photosynthesis
It goes up and plateaus
What happens in the time of day graph of rate of photosynthesis
At 00:00 it start going up
At 12:00 it peak and dips down again for 24:00
What happens in the carbon dioxide concentration graph of rate of photosynthesis
Goes up and then keeps going up very vey slightly
What happens in the termperature graph of rate of photosynthesis
Goes up really high then goes all the way down
What are the limiting factors in the light intensity graph of rate of photosynthesis which has the letters A, B and C and 2 lines based on higher and lower CO2
A - Light intensity
B - carbon dioxide
C - Temperature
How is maximum heat achieved in a green house
Paraffin heaters
Artificial light
Water sprinklers
Fertilisers
Give the word equation for respiration
Glucose + oxygen -> water + carbon dioxide + energy
Whenever both respiration and photosynthesis are equal what is it called
Compensation point
Explain why raising light intensity increases the rate of photosynthesis
As light is an essential part of photosynthesis
What is a heat shield
A heat shield means there is only one source of heat.
When are compensation points
Dawn and dusk
Describe photosynthesis and respiration during the day
Both photosynthesis and respiration occur
At high light intensity (midday) - the rate of photosynthesis is greater than the rate of respiration
Therefore the net movement is CO2 enters and O2 exits the leaf
Describe photosynthesis and respiration at night
There is no light for photosynthesis
Respiration still occurs
So O2 enters the leaf and CO2 leaves it
Describe photosynthesis and respiration at low light intensity (dawn and dusk)
The rate of photosynthesis slows down and is equal to the rate of respiration. This is the compensation point. Therefore overall there is no gas exchange as:
The CO2 produced in respiration is used for photosynthesis
the O2 produced in photosynthesis is used for respiration
How can the movement of carbon dioxide and oxygen into and out of plants be determined
Using hydrogen carbonate indicator
Give the three colours of hydrogen carbonate indicator and why
Red in normal atmospheric carbon dioxide levels
Yellow in increased carbon dioxide levels
Purple in decreased carbon dioxide levels
What are the main factors of photosynthesis and crop production
Temperature Carbon dioxide levels Light intensity Mineral availability Water availability
Which factor limits photosynthesis on a bright winter afternoon in a British grassland
There will be enough light and carbon dioxide therefore temperature is likely to be limiting
What factor is limiting photosynthesis in a cornfield in mid summer sunshine in southern France
There will be enough light and temperatures will be high. Carbon dioxide is likely to be the limiting factor
On a graph with Y axis (rate of photosynthesis) what are the limiting factors at A, B and C
A - light intensity
B - temperature
C - carbon dioxide
Why do thin leaves help light absorption
The short distance from the top of a leaf (cuticle) to the bottom (lower epidermis) allows all the cells to receive light
Why does a large surface area help light absorption
Enables the leaf to absorb maximum sunlight onto its surface
Why do cuticles help light absorption
This is thin and transparent so doesn’t prevent light entering, but is also a waxy layer so also reduces water loss by evaporation. It is a physical defence mechanism
Why does a palisade mesophyll layer help light absorption
Contains many tightly packed and regular shaped palisade cells that are rich in chloroplasts near the surface of the leaf to obtain maximum sunlight
What does the upper epidermis do to help light absorption
Cell walls are a physical defence for leaf against microbes, and are transparent to allow light to pass through
What do chloroplasts do to help light absorption
Contain the pigment chlorophyll to absorb sunlight
What does a spongy mesophyll layer do to help gas exchange
The cells have fewer chloroplasts and are irregularly shaped resulting in a large surface area for gas exchange. There are many air spaces within this layer
What do intercellular air spaces do to help gas exchange
These occur in the spongy mesophyll and allow the movement of oxygen and carbon dioxide through the leaf
What do guard cells and stomata do to help gas exchange
Carbon dioxide and oxygen can enter and leave through the stomata. Stomata are small pores that occur between the guard cells. These guard cells regulate the opening and closing of stomata. There are more stomata non the underside of a mesophytic leaf to prevent water loss. In many plants stomata are open during the day but closes at night
How did the leaf change when placed in boiling water - what has happened and why
When placed I’m boiling water the plant was softened and killed as the temperature was so high. It also allows chemicals to enter
How did the leaf change when it was placed in boiling ethanol - why is this an important step
When placed in boiling ethanol all of the chlorophyll came out
In the experiment finding out if chlorophyll is needed for photosynthesis what colour changes occur to the leaf and why
Only the green parts of the variegated leaf contained chlorophyll and so they reacted with the iodine and turned blueblack. The areas without chlorophyll are unable to produce starch so they do not turn blueblack
How is a plant destarched
Leave in a dark place for a period of time
Why is a plant destarched
To prevent photosynthesis
How much starch can be stored in a plant
Unlimited as it is insoluble and so doesn’t upset the water balance of the cells.
What can glucose be turned into in a plant
- Sucrose and carried to other parts of the plant
- Glucose for respiration or active transport
- Starch and stored
- Glucose combines with nitrates to become amino acids which are made into protein - a vital building block for plant cells involved in growth and repair
- Lipids for storage in seeds
Why is glucose turned into lipids in seeds
Lipids are insoluble and contain twice as much energy as the same weight of starch, thus enabling storage of more energy in a small space.
How do you adapt the elodea test to show how light intensity, carbon dioxide and temperature affects the rate of photosynthesis
Light intensity- by moving a desk lamp further away at pre determined intervals from the pond weed
Carbon dioxide - by adding more spatulas of hydrogen carbonate indicator to the water
Temperature - by adding warm water to the experiment and recording the temperature with a thermometer
Why is it necessary to take 5 minutes before taking measurements in the elodea test
Need plant to get ready for new conditions and get used to it (plant needs to equilibrate to new conditions)