Garlic and Mint on Bacterial Growth (sort this out)

Question 1

Independent variable.

Answer 1

presence of garlic or mint

Question 2

Dependent variable.

Answer 2

area of zone of inhibition around disk

Question 3

Other variables

Answer 3

• concentration of plant material
• lawn of bacteria on petri dish
• contamination of petri dish by other microbes
• same volume of plant material on each disc

Question 4

Equipment

Answer 4

• Agar plate seeses with bacteria
• plant material
• pestle and mortar
• 10cm3 denatured alcohol
• sterile pipette
• paper discs
• sterile petri dish
• sterile forceps

Question 5

Method

Answer 5

1. make plant extract by crushing 3g of plant material with 10cm3 of alcohol - shake ocasionally for 10 mins
2. pipette 0.1cm3 of extract onto sterile paper disc and allow to dry
   3.