Frozen -> Thawed Deglycerolized RBCs Flashcards
Indications
- anemia
- long term storage of rare units
Rare units
“AB negative”
Rh phenotype
Bombay phenotype
autologous donation
cryoprotective agent to store the specimen at a very low temperature without destructing the red blood cells
glycerol
% of High concentration glycerol
40%
High conc glycerol storage
mechanical freezer
-65 deg C
% of Low concentration glycerol
20%
Low conc glycerol storage
liquid nitrogen
-120 deg C
Formation of ice crystals that leads to cell lysis
Low concentration glycerol
79% glycerol with dextrose fructose and EDTA
-65 deg C
Shelf Life:
Frozen ____ , add a cryoprotective agent
Deglycerolized Thawed _____
10 years
24hrs
hypertonic; removes glycerol without affecting
the viability of the red blood cells
12% NaCl
facilitates the complete removal of glycerol
1.6% NaCl
isotonic solution; required for red blood cell washing prior to transfusion
0.85% NaCl
0.2% dextrose in normal saline
maintains the normal morphology of the RBC prior to transfusion
NSS
used to provide ATP for cell consumption
Dextrose
utilizes low molecular weight agent
Penetrating Cryoprotective Agent
utilizes high molecular weight agent
Non-Penetrating Cryoprotective Agent
commonly used Penetrating Cryoprotective Agent
Glycerol
Commonly used Non-Penetrating Cryoprotective Agent
HES
Actions of the Penetrating Cryoprotective Agent
Protect cell membrane
Prevents ice crystal formation
Prevents dehydration
Action of Penetrating Non-Cryoprotective Agent
protects cell membrane
