Frozen -> Thawed Deglycerolized RBCs Flashcards
Indications
- anemia
- long term storage of rare units
Rare units
“AB negative”
Rh phenotype
Bombay phenotype
autologous donation
cryoprotective agent to store the specimen at a very low temperature without destructing the red blood cells
glycerol
% of High concentration glycerol
40%
High conc glycerol storage
mechanical freezer
-65 deg C
% of Low concentration glycerol
20%
Low conc glycerol storage
liquid nitrogen
-120 deg C
Formation of ice crystals that leads to cell lysis
Low concentration glycerol
79% glycerol with dextrose fructose and EDTA
-65 deg C
Shelf Life:
Frozen ____ , add a cryoprotective agent
Deglycerolized Thawed _____
10 years
24hrs
hypertonic; removes glycerol without affecting
the viability of the red blood cells
12% NaCl
facilitates the complete removal of glycerol
1.6% NaCl
isotonic solution; required for red blood cell washing prior to transfusion
0.85% NaCl
0.2% dextrose in normal saline
maintains the normal morphology of the RBC prior to transfusion
NSS
used to provide ATP for cell consumption
Dextrose
