Formulae (L3)

Question 1

Formula for Determination of the Object magnification (OM) for LM visual magnification?

Answer 1

Object magnification = IDo / ODo

Question 2

IDo?

Answer 2

Image distance (for object magnification).

Question 3

ODo?

Answer 3

Object distance (for object magnification).

Question 4

What attributes does Image formation involve? (2)

Answer 4

• LM image formation.
• Determination of magnification.

Question 5

Types of image formation in LM? (2)

Answer 5

• Visual image formation.
• Projection image formation.

Question 6

What is projection image formation used for?

Answer 6

Used for the image projection & photography/display on LCD monitors.

Question 7

Formula for Determination of the Eyepiece magnification (EM) for LM visual magnification?

Answer 7

Eyepiece magnification = Shortest distance of distinct vision/ ODe

Question 8

ODe?

Answer 8

Object distance (for eyepiece magnification).

Question 9

Formula for Determination of the Total magnification (TM) for LM visual magnification?

Answer 9

Total magnification = OM × EM

Question 10

How to determine Field Of View (FOV) [Steps]? (3)

Answer 10

● Use transparent ruler to measure diameter of your FOV from under the microscope for different magnifications (in mm).

● Convert mm to μm.

● Fill in table [Total magnification; FOV (mm); FOV (μm)].

Question 11

Relationship between Total magnification & FOV?

Answer 11

As magnification increases, FOV decreases.

Question 12

FOV formulae? (2)

Answer 12

● Actual size of specimen equation.

● Scale bar equation.

Question 13

Actual size of specimen formula?

Answer 13

Actual size of specimen = FOV (μm) / How many times specimen fits on FOV

Question 14

Scale bar formula?

Answer 14

Scale bar = Actual size / Size of drawing (“diameter”) on paper (cm)

Question 15

Formula for Determination of the Object magnification (OM) for LM projection magnification?

Answer 15

OM = IDo / ODo.

Question 16

Formula for Determination of the Project lens magnification (PLM) for LM projection magnification?

Answer 16

PLM = IDp / ODp

Question 17

IDp?

Answer 17

Image distance (for PLM).

Question 18

ODp?

Answer 18

Object distance (for PLM).

Question 19

Formula for Determination of the Total magnification (TM) for LM projection magnification?

Answer 19

TM = OM × PLM

Question 20

Equation for the Numerical Aperture (N.A.)?

Answer 20

N.A. objective = sinα × R.I

Question 21

R.I stands for?

Answer 21

Refractive Index.

Question 22

Equation for Resolution Power (RP) of microscope?

Answer 22

RP of microscope = 0.61λ / N.A.

Question 23

λ?

Answer 23

= wavelength.