FoC Thermodynamics

Question 1

What’s Kd

Answer 1

[PL]/[P][L]

Question 2

What does stronger affinity mean

Answer 2

Smaller Kd and larger Ka

Question 3

What is fractional occupancy in binding affinity

Answer 3

B = [PL]/[P] + [PL]

Question 4

What is Langmuir isotherm

Answer 4

B as a function of [L]
B = [L] / [L] + [Kd]

Question 5

What the linearized Langmuir isotherm

Answer 5

LnB/1-B = ln[L] - ln[Kd]

X axis = ln [L]
Y axis = ln[b/b-1]
Gradient =1
Intercept = -ln [Kd]

Question 6

Fractional occupancy in binding stiochiometry

Answer 6

B= [PLn] / [P] + [PLn]

Question 7

Protein ligand binding reaction

Answer 7

P + nL both ways arrow PLn

Question 8

What is the hill isotherm

Answer 8

B= [L] / Kd + [L]

Question 9

Linearised hill isotherm

Answer 9

Ln(b/b-1) = n ln [L] + ln (Kd)
X axis = ln [L]
Y axis = ln(B/1-B)
Gradient = n
Intercept = -ln[Kd]

Question 10

Out of Wp+l and Wpl which has a higher enthalpy

Answer 10

Wp+l

Question 11

What is the dissociation constant from the Boltzmann

Answer 11

Kd= Wp+l/Wpl e -Wp+l - Wpl/RT

Question 12

Entropy of dissociation

Answer 12

Delta Sd = R ln Wp+l/Wpl

Question 13

What is the entropy of Sp+l

Answer 13

R ln Wp+l

Question 14

What is the entropy of PL

Answer 14

Spl = R ln Wpl

Question 15

Hobbs free energy

Answer 15

Delta G = delta H - T delta S

Question 16

Delta g in terms of K

Answer 16

Delta g = -RT ln k

Question 17

What is Van Hoff plot

Answer 17

Ln K = - delta H/R x 1/T + delta S /R
X axis = 1/T
Y axis = ln K
Gradient = -delta H / R
Intercept = delta s / R

Question 18

the equation for calorimetry of protein

Answer 18

deltaCp = Cp(water+protein)- Cp(water)

Question 19

what shape is the denaturation graph

Answer 19

bell curve with a right lean

Question 20

how to calculate delta Hm from denaturation graph

Answer 20

the area under the graph subtracking the baseline