FoC Thermodynamics Flashcards
What’s Kd
[PL]/[P][L]
What does stronger affinity mean
Smaller Kd and larger Ka
What is fractional occupancy in binding affinity
B = [PL]/[P] + [PL]
What is Langmuir isotherm
B as a function of [L]
B = [L] / [L] + [Kd]
What the linearized Langmuir isotherm
LnB/1-B = ln[L] - ln[Kd]
X axis = ln [L]
Y axis = ln[b/b-1]
Gradient =1
Intercept = -ln [Kd]
Fractional occupancy in binding stiochiometry
B= [PLn] / [P] + [PLn]
Protein ligand binding reaction
P + nL both ways arrow PLn
What is the hill isotherm
B= [L] / Kd + [L]
Linearised hill isotherm
Ln(b/b-1) = n ln [L] + ln (Kd)
X axis = ln [L]
Y axis = ln(B/1-B)
Gradient = n
Intercept = -ln[Kd]
Out of Wp+l and Wpl which has a higher enthalpy
Wp+l
What is the dissociation constant from the Boltzmann
Kd= Wp+l/Wpl e -Wp+l - Wpl/RT
Entropy of dissociation
Delta Sd = R ln Wp+l/Wpl
What is the entropy of Sp+l
R ln Wp+l
What is the entropy of PL
Spl = R ln Wpl
Hobbs free energy
Delta G = delta H - T delta S
Delta g in terms of K
Delta g = -RT ln k
What is Van Hoff plot
Ln K = - delta H/R x 1/T + delta S /R
X axis = 1/T
Y axis = ln K
Gradient = -delta H / R
Intercept = delta s / R
the equation for calorimetry of protein
deltaCp = Cp(water+protein)- Cp(water)
what shape is the denaturation graph
bell curve with a right lean
how to calculate delta Hm from denaturation graph
the area under the graph subtracking the baseline
