Fluorometry

Question 1

Fluorescence

Answer 1

Ability to absorb light of high energy, shorter wavelengths and emits it at a lower energy, longer wavelengths

Question 2

Fluorophore

Answer 2

A fluorescent chemical compound

Question 3

principle of fluorometry

Answer 3

measure concentration of solutions that contain fluorescing molecules

as molecules/ atoms absorb energy , electrons become excited

higher energy levels ( excited electrons)
electrons to ground state & emit light

if emitted wavelength is longer than absorbed for excitation ( excitation wavelength) = fluorescence